Home LiteratureArticle Details
PMID: 6251277 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Evidence for the common origin of viral and cellular sequences involved in sarcomagenic transformation.

Journal of virology ·Vol. 35 ·No. 1 ·1980-07-00 ·Pages 52-64

Wang LH, Snyder P, Hanafusa T, Hanafusa H

Abstract

The src genes of six different strains of avian sarcoma virus (ASV) were compared with those of a series of newly isolated sarcoma viruses, termed "recovery avian sarcoma viruses" (rASV's). The rASV's were isolated recently from chicken and quail tumors induced by transformation-defective (td) deletion mutants of Schmidt-Ruppin Rous sarcoma virus. The RNase T1-resistant oligonucleotide maps were constructed for the RNA genomes of different strains of ASV and td mutants. The src-specific sequences, characterized by RNase T1-resistant oligonucleotides ranging from 9 to 19 nucleotides long, were defined as those mapping between approximately 600 and 2,800 nucleotides from the 3' polyadenylate end of individual sarcoma viral RNAs, and missing in the corresponding td viral RNAs. Our results revealed that 12 src-specific oligonucleotides were highly conserved among several strains of ASV, including the rASV's, whereas certain strains of ASV were found to contain one to three characteristic src-specific oligonucleotides. We previously presented evidence supporting the idea that most of the src-specific sequences present in rASV RNAs are derived from cellular genetic information. Our present data indicate that the src genes of rASV's are closely related to other known ASVs. We conclude that the src genes of different strains of ASV and the cellular sarc sequences are of common origin, although some divergence has occurred among different viral src genes and related cellular sequences.

MeSH Terms
Alpharetrovirus/genetics Animals Avian Sarcoma Viruses/genetics Base Sequence Cell Transformation, Viral Chick Embryo Chromatography Electrophoresis Genes, Viral RNA, Viral/analysis
Chemicals
RNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wang L H
Snyder P
Hanafusa T
Hanafusa H
References (53)
53 references, click to expand
  1. Oncogenes of RNA tumor viruses as determinants of cancer.
    Proc Natl Acad Sci U S A. 1969 Nov;64(3):1087-94 PMID: 5264139
  2. N.A.S. symposium: new evidence as the basis for increased efforts in cancer research.
    Proc Natl Acad Sci U S A. 1972 Apr;69(4):1009-15 PMID: 4554531
  3. The RNA tumor viruses--background and foreground.
    Proc Natl Acad Sci U S A. 1972 Apr;69(4):1016-20 PMID: 4337233
  4. Genetic recombination with avian tumor virus.
    Virology. 1972 Jul;49(1):37-44 PMID: 4338996
  5. Electrophoretic analysis of the RNA of avian tumor viruses.
    Virology. 1972 Dec;50(3):753-64 PMID: 4344943
  6. Studies on the nucleic acid sequences of Kirsten sarcoma virus: a model for formation of a mammalian RNA-containing sarcoma virus.
    J Virol. 1973 Sep;12(3):458-63 PMID: 4127029
  7. Carcinogenesis by RNA sarcoma viruses. XII. A quantitative study of infection of rat cells in vitro by avian sarcoma viruses.
    Virology. 1970 Jan;40(1):118-34 PMID: 4312371
  8. Avian tumor virus RNA: a comparison of three sarcoma viruses and their transformation-defective derivatives by oligonucleotide fingerprinting and DNA-RNA hybridization.
    Proc Natl Acad Sci U S A. 1973 Aug;70(8):2266-70 PMID: 4365699
  9. Properties and location of poly(A) in Rous sarcoma virus RNA.
    J Virol. 1974 Dec;14(6):1515-29 PMID: 4372409
  10. RNA of replication-defective strains of Rous sarcoma virus.
    Proc Natl Acad Sci U S A. 1975 Apr;72(4):1569-73 PMID: 165514
  11. Mapping RNase T1-resistant oligonucleotides of avian tumor virus RNAs: sarcoma-specific oligonucleotides are near the poly(A) end and oligonucleotides common to sarcoma and transformation-defective viruses are at the poly(A) end.
    J Virol. 1975 Oct;16(4):1051-70 PMID: 170411
  12. Mapping of biological functions on RNA of avian tumor viruses: location of regions required for transformation and determination of host range.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4772-6 PMID: 174075
  13. Location of envelope-specific and sarcoma-specific oligonucleotides on RNA of Schmidt-Ruppin Rous sarcoma virus.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):447-51 PMID: 174108
  14. A physical map of the Rous sarcoma virus genome.
    J Mol Biol. 1976 Jan 25;100(3):293-318 PMID: 176365
  15. Purification of DNA complementary to nucleotide sequences required for neoplastic transformation of fibroblasts by avian sarcoma viruses.
    J Mol Biol. 1976 Mar 5;101(3):349-65 PMID: 176368
  16. Distribution of envelope-specific and sarcoma-specific nucleotide sequences from different parents in the RNAs of avian tumor virus recombinants.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1073-7 PMID: 177972
  17. Independent regulation of endogenous and exogenous avian RNA tumor virus genes.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2259-63 PMID: 181750
  18. Nucleotide sequences in mouse DNA and RNA specific for Moloney sarcoma virus.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3705-9 PMID: 185623
  19. Mapping oligonucleotides of Rous sarcoma virus RNA that segregate with polymerase and group-specific antigen markers in recombinants.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3952-6 PMID: 186781
  20. Purification of DNA complementary to the env gene of avian sarcoma virus and analysis of relationships among the env genes of avian leukosis-sarcoma viruses.
    J Virol. 1977 Feb;21(2):497-505 PMID: 189084
  21. Heteroduplex analysis of avian RNA tumor viruses.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):477-81 PMID: 191811
  22. A new replication-defective variant of the Bryan high-titer strain Rous sarcoma virus.
    Virology. 1977 Apr;77(2):705-21 PMID: 67703
  23. Size and genetic content of viral RNAs in avian oncovirus-infected cells.
    J Virol. 1977 Oct;24(1):47-63 PMID: 198585
  24. Genetic variation in the RNA transcripts of endogenous virus genes in uninfected chicken cells.
    J Virol. 1977 Oct;24(1):64-73 PMID: 198586
  25. Identification of a transformation-specific antigen induced by an avian sarcoma virus.
    Nature. 1977 Sep 22;269(5626):346-8 PMID: 198667
  26. The terminal oligonucleotides of avian tumor virus RNAs are genetically linked.
    Virology. 1977 Oct 15;82(2):472-92 PMID: 199998
  27. Recovery of avian sarcoma virus from tumors induced by transformation-defective mutants.
    J Exp Med. 1977 Dec 1;146(6):1735-47 PMID: 200700
  28. Occurrence of partial deletion and substitution of the src gene in the RNA genome of avian sarcoma virus.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4781-5 PMID: 200931
  29. Transformation-defective mutants of Rous sarcoma virus with src gene deletions of varying length.
    J Virol. 1977 Dec;24(3):910-4 PMID: 201783
  30. Uninfected avian cells contain RNA related to the transforming gene of avian sarcoma viruses.
    Cell. 1978 Feb;13(2):371-9 PMID: 203404
  31. Protein kinase activity associated with the avian sarcoma virus src gene product.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4 PMID: 205879
  32. Identification of a polypeptide encoded by the avian sarcoma virus src gene.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1567-71 PMID: 206909
  33. In vitro synthesis of a functional avian sarcoma virus transforming-gene product.
    Nature. 1978 Aug 31;274(5674):919-21 PMID: 210399
  34. The gene order of avian RNA tumor viruses derived from biochemical analyses of deletion mutants and viral recombinants.
    Annu Rev Microbiol. 1978;32:561-92 PMID: 213012
  35. Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoprotein.
    Cell. 1978 Oct;15(2):561-72 PMID: 214242
  36. Characterization of a normal avian cell protein related to the avian sarcoma virus transforming gene product.
    Cell. 1978 Dec;15(4):1363-9 PMID: 83198
  37. Recombination between viral and cellular sequences generates transforming sarcoma virus.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):5812-6 PMID: 215998
  38. Src Gene product from different strains of avian sarcoma virus: Kinetics and possible mechanism of heat inactivation of protein kinase activity from cells infected by transformation-defective, temperature-sensitive mutant and wild-type virus.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):967-71 PMID: 218225
  39. Characterization of some isolates of newly recovered avian sarcoma virus.
    J Virol. 1979 Jan;29(1):91-101 PMID: 219237
  40. Detection of the viral sarcoma gene product in cells infected with various strains of avian sarcoma virus and of a related protein in uninfected chicken cells.
    J Virol. 1979 Mar;29(3):1196-203 PMID: 221673
  41. Uninfected vertebrate cells contain a protein that is closely related to the product of the avian sarcoma virus transforming gene (src).
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1804-8 PMID: 221907
  42. Restitution of fibroblast-transforming ability in src deletion mutants of avian sarcoma virus during animal passage.
    Virology. 1979 Mar;93(2):413-26 PMID: 222047
  43. Specific RNA sequences of Rous sarcoma virus (RSV) recovered from tumors induced by transformation-defective RSV deletion mutants.
    Virology. 1979 Mar;93(2):427-34 PMID: 222048
  44. Polymorphism of avian sarcoma virus src proteins.
    J Virol. 1979 Apr;30(1):190-200 PMID: 225515
  45. Product of in vitro translation of the Rous sarcoma virus src gene has protein kinase activity.
    J Virol. 1979 Apr;30(1):311-8 PMID: 225522
  46. Suppression of multiplication of avian sarcoma virus by rapid spread of transformation-defective virus of the same subgroup.
    J Virol. 1979 Aug;31(2):389-97 PMID: 225561
  47. Cellular information in the genome of recovered avian sarcoma virus directs the synthesis of transforming protein.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3154-8 PMID: 226955
  48. A normal cell protein similar in structure and function to the avian sarcoma virus transforming gene product.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3159-63 PMID: 226956
  49. Analysis of the src gene of sarcoma viruses generated by recombination between transformation-defective mutants and quail cellular sequences.
    J Virol. 1979 Nov;32(2):546-56 PMID: 228078
  50. Identification of a Rous sarcoma virus transformation-related protein in normal avian and mammalian cells.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4479-83 PMID: 92031
  51. Comparative analysis of cellular and viral sequences related to sarcomagenic cell transformation.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 2,:755-64 PMID: 6253210
  52. The defectiveness of Rous sarcoma virus.
    Proc Natl Acad Sci U S A. 1963 Apr;49:572-80 PMID: 13952461
  53. Quantitative studies on Rous sarcoma virus. V. An analysis of the mechanism of virulence of the Bryan "high titer" strain of RSV.
    Virology. 1960 Jun;11:371-99 PMID: 14434915
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.35.1.52-64.1980
Published
1980-07-00
Pages
52-64
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288782
Subset
IM
Grants
NCI NIH HHS · CA00574 · United States
NCI NIH HHS · CA14935 · United States
NCI NIH HHS · T32CA09256 · United States
External Links
PubMed source DOI: 10.1128/JVI.35.1.52-64.1980 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2