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PMID: 6205164 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Monoclonal antibodies to Rous sarcoma virus pp60src react with enzymatically active cellular pp60src of avian and mammalian origin.

Journal of virology ·Vol. 51 ·No. 2 ·1984-08-00 ·Pages 272-82

Parsons SJ, McCarley DJ, Ely CM, Benjamin DC, Parsons JT

Abstract

The derivation and characterization of 22 hybridoma clones producing monoclonal antibodies (Mabs) specific for the transforming protein of Rous sarcoma virus, pp60src, are described. All Mabs reacted with pp60v-src encoded by Prague, Schmidt-Ruppin, and Bratislava 77 strains of Rous sarcoma virus. Of these Mabs, 10 efficiently immunoprecipitated pp60c-src from chicken embryo cells. Of these 10 Mabs, 2 (GD11 and EB8) readily detected pp60c-src from a variety of rodent and human cultured cells and from rat brain tissue in an in vitro immune complex kinase assay. Mapping experiments have tentatively localized the determinant(s) recognized by GD11 and EB8 to a region of the src protein bounded by amino acid residues 82 to 169, whereas the remaining Mabs appeared to recognize determinants residing within residues 1 to 82 or 169 to 173. Most of the Mabs complexed denatured pp60v-src in a Western immunoblot, and several were used to localize pp60v-src in Rous sarcoma virus-transformed chicken embryo cells by indirect immunofluorescence microscopy.

MeSH Terms
Animals Antibodies, Monoclonal/isolation & purification Antigen-Antibody Complex/analysis Avian Sarcoma Viruses/enzymology Cells, Cultured Chick Embryo Cross Reactions Electrophoresis, Polyacrylamide Gel Epitopes/analysis Fluorescent Antibody Technique Immunoglobulins/isolation & purification Mice Mice, Inbred BALB C Oncogene Protein pp60(v-src) Plasmids Protein Kinases/metabolism Protein-Tyrosine Kinases Viral Proteins/immunology,metabolism
Chemicals
Antibodies, Monoclonal Antigen-Antibody Complex Epitopes Immunoglobulins Viral Proteins Protein Kinases Protein-Tyrosine Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Parsons S J
McCarley D J
Ely C M
Benjamin D C
Parsons J T
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38 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.51.2.272-282.1984
Published
1984-08-00
Pages
272-82
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254436
Subset
IM
Grants
NCI NIH HHS · CA27578 · United States
NCI NIH HHS · CA29243 · United States
External Links
PubMed source DOI: 10.1128/JVI.51.2.272-282.1984 PMC Full Text View journal details
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