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PMID: 6251464 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Adhesion plaques of Rous sarcoma virus-transformed cells contain the src gene product.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 6 ·1980-06-00 ·Pages 3514-8

Rohrschneider LR

Abstract

Another intracellular location of the Rous sarcoma virus (RSU) src gene product (pp60src) has been detected within RSV-transformed cells by indirect immunofluorescence. By using rabbit anti-tumor serum specific for pp60src, a speckled pattern of fluorescence was found on the ventral surface of RSV (Schmidt-Ruppin strain)-transformed normal rat kidney cells. Several tests indicated that this pattern was specific for pp60src. In addition, interference-reflection microscopy was used to visualize cellular adhesion plaques, which are the points at which cells attach to the substratum. Simultaneous immunofluorescence and interference-reflection microscopy indicated that the speckles of pp60src fluorescence corresponded exactly to the adhesion plaque structures. The presence of pp60src within the adhsion plaques was further demonstrated by indirect immunofluorescences on isolated adhesion plaques that remained bound to glass after removal of the cells. pp60src also was observed in adhesion plaques of RSV-tranformed chicken embryo fibroblasts (CEF) and mouse fibroblasts, as well as CEF infected with the temperature-sensitive RSV mutant tsNY68 and grown at permissive temperature. At nonpermissive temperature, pp60src was not detectable in adhesion plaques of the tsNY68-infected CEF. Adhesion plaques serve as focal points of microfilament bundle attachment, and thse results suggest that pp60src interacts directly with cellular cytoskeletal components.

MeSH Terms
Animals Avian Sarcoma Viruses/enzymology,genetics Cell Adhesion Cell Membrane/analysis Cell Transformation, Viral Cells, Cultured Chick Embryo Cytoskeleton/metabolism Fluorescent Antibody Technique Genes, Viral Kidney/cytology Mice Microscopy, Interference Mutation Neoplasm Proteins/analysis Protein Kinases/analysis Rats Temperature Viral Proteins/analysis
Chemicals
Neoplasm Proteins Viral Proteins Protein Kinases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Rohrschneider L R
References (28)
28 references, click to expand
  1. Identification of a Rous sarcoma virus transformation-related protein in normal avian and mammalian cells.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4479-83 PMID: 92031
  2. Localization of the ASV src gene product to the plasma membrane of transformed cells by electron microscopic immunocytochemistry.
    Cell. 1979 Sep;18(1):125-34 PMID: 228858
  3. A 130K protein from chicken gizzard: its localization at the termini of microfilament bundles in cultured chicken cells.
    Cell. 1979 Sep;18(1):193-205 PMID: 574428
  4. Three-dimensional organization of microfilaments and microtubules in the cytoskeleton. Immunoperoxidase labelling and stereo-electron microscopy of detergent-extracted cells.
    Exp Cell Res. 1979 Dec;124(2):301-16 PMID: 389645
  5. Evidence that the avian sarcoma virus transforming gene product is a cyclic AMP-independent protein kinase.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6260-4 PMID: 230504
  6. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 PMID: 6246487
  7. Identification of a transformation-specific antigen induced by an avian sarcoma virus.
    Nature. 1977 Sep 22;269(5626):346-8 PMID: 198667
  8. Cell to substratum contacts of chick fibroblasts and their relation to the microfilament system. A correlated interference-reflexion and high-voltage electron-microscope study.
    J Cell Sci. 1978 Feb;29:197-212 PMID: 564353
  9. Protein kinase activity associated with the avian sarcoma virus src gene product.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4 PMID: 205879
  10. Identification of a polypeptide encoded by the avian sarcoma virus src gene.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1567-71 PMID: 206909
  11. Assay for early cytoplasmic effects of the src gene product of Rous sarcoma virus.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2750-4 PMID: 208075
  12. In vitro synthesis of a functional avian sarcoma virus transforming-gene product.
    Nature. 1978 Aug 31;274(5674):919-21 PMID: 210399
  13. The gene order of avian RNA tumor viruses derived from biochemical analyses of deletion mutants and viral recombinants.
    Annu Rev Microbiol. 1978;32:561-92 PMID: 213012
  14. Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoprotein.
    Cell. 1978 Oct;15(2):561-72 PMID: 214242
  15. Characterization of the avian sarcoma virus protein p60src.
    Virology. 1978 Nov;91(1):130-40 PMID: 214955
  16. Relationships between fibronectin (LETS protein) and actin.
    Cell. 1978 Nov;15(3):875-86 PMID: 365353
  17. Immunofluorescence on avian sarcoma virus-transformed cells: localization of the src gene product.
    Cell. 1979 Jan;16(1):11-24 PMID: 217542
  18. Structural analysis of the avian sarcoma virus transforming protein: sites of phosphorylation.
    J Virol. 1979 Feb;29(2):770-81 PMID: 219258
  19. Product of in vitro translation of the Rous sarcoma virus src gene has protein kinase activity.
    J Virol. 1979 Apr;30(1):311-8 PMID: 225522
  20. Characterization of the protein kinase activity of avian sarcoma virus src gene product.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5028-32 PMID: 228274
  21. THE MECHANISM OF ADHESION OF CELLS TO GLASS. A STUDY BY INTERFERENCE REFLECTION MICROSCOPY.
    J Cell Biol. 1964 Feb;20:199-215 PMID: 14126869
  22. The effects of reciprocal changes in temperature on the transformed state of cells infected with a rous sarcoma virus mutant.
    Virology. 1971 Nov;46(2):470-9 PMID: 4331732
  23. Electronmicroscope investigations of the underside of cells in culture.
    Exp Cell Res. 1973 Mar 30;78(1):1-14 PMID: 4570581
  24. Adhesions of fibroblasts to substratum during contact inhibition observed by interference reflection microscopy.
    Exp Cell Res. 1975 Apr;92(1):57-62 PMID: 1169157
  25. Cell-to-substrate contacts in living fibroblasts: an interference reflexion study with an evaluation of the technique.
    J Cell Sci. 1976 Jun;21(1):129-59 PMID: 932106
  26. Reversion from transformed to normal phenotype by inhibition of protein synthesis in rat kidney cells infected with a temperature-sensitive mutant of Rous sarcoma virus.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3603-7 PMID: 185620
  27. Decreased adherence to the substrate in Rous sarcoma virus-transformed chicken embryo fibroblasts.
    Cell. 1977 Jan;10(1):45-51 PMID: 189934
  28. Microfilament bundles and cell shape are related to adhesiveness to substratum and are dissociable from growth control in cultured fibroblasts.
    Cell. 1977 Mar;10(3):375-80 PMID: 557370
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.6.3514
Published
1980-06-00
Pages
3514-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349647
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.6.3514 PMC Full Text View journal details
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