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PMID: 6401819 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Development of anti-pp60src serum with antigen produced in Escherichia coli.

Journal of virology ·Vol. 45 ·No. 1 ·1983-01-00 ·Pages 462-5

Gilmer TM, Erikson RL

Abstract

We have purified p60(src) from bacterial recombinants which direct the synthesis of the Rous sarcoma virus transforming gene (src) product. This protein was injected into rabbits, and they produced a highly cross-reactive serum which can recognize the src protein from many different strains of Rous sarcoma virus.

MeSH Terms

Cross Reactions DNA, Recombinant Escherichia coli/genetics,metabolism Immune Sera/immunology Oncogene Protein pp60(v-src) Viral Proteins/biosynthesis,immunology

Chemicals

DNA, Recombinant Immune Sera Viral Proteins Oncogene Protein pp60(v-src)

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Gilmer T M

Erikson R L

References (10)

10 references, click to expand

Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
Identification of a transformation-specific antigen induced by an avian sarcoma virus.
Nature. 1977 Sep 22;269(5626):346-8 PMID: 198667
Protein kinase activity associated with the avian sarcoma virus src gene product.
Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4 PMID: 205879
Construction of plasmids for expression of Rous sarcoma virus transforming protein, p60src, in Escherichia coli.
Proc Natl Acad Sci U S A. 1982 Apr;79(7):2152-6 PMID: 6285367
A normal cell protein similar in structure and function to the avian sarcoma virus transforming gene product.
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3159-63 PMID: 226956
Plasmids of Escherichia coli as cloning vectors.
Methods Enzymol. 1979;68:245-67 PMID: 232214
Protein phosphorylation mediated by partially purified avian sarcoma virus transforming-gene product.
Cold Spring Harb Symp Quant Biol. 1980;44 Pt 2,:907-17 PMID: 6253222
Rous sarcoma virus transforming protein, p60src, expressed in E. coli, functions as a protein kinase.
Nature. 1981 Dec 24;294(5843):771-3 PMID: 6172716
Structural analysis of the avian sarcoma virus transforming protein: sites of phosphorylation.
J Virol. 1979 Feb;29(2):770-81 PMID: 219258

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.45.1.462-465.1983

Published

1983-01-00

Pages

462-5

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC256431

Subset

Grants

NCI NIH HHS · CA 06839 · United States

NCI NIH HHS · CA-15823 · United States

NCI NIH HHS · CA-21117 · United States

External Links

PubMed source DOI: 10.1128/JVI.45.1.462-465.1983 PMC Full Text View journal details

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