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PMID: 6328280 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Tyrosyl and cyclic AMP-dependent protein kinase activities in BHK cells that express viral pp60src.

Molecular and cellular biology ·Vol. 4 ·No. 5 ·1984-05-00 ·Pages 973-7

Clinton GM, Roskoski R

Abstract

Protein kinase activities were measured in Rous sarcoma virus-infected baby hamster kidney (BHK) cells that express v-src (BHK [v-src]) and compared with those of revertant and control BHK cells. We observed about a fivefold-higher tyrosine phosphorylating activity in BHK (v-src) cell extracts, which was due to src but not other cellular tyrosyl kinase activities since preincubation with anti-src serum reduced the activity to control cell levels. The cyclic AMP-dependent protein kinase activity was also altered when v-src was expressed. Resolution of the two cyclic AMP-dependent isozymes from the detergent-soluble fraction of cells revealed that the type I activity was selectively decreased about fivefold in BHK (v-src) cells.

MeSH Terms
Animals Avian Sarcoma Viruses/enzymology,genetics Cell Line Cell Transformation, Viral Cricetinae Kidney Kinetics Oncogene Protein pp60(v-src) Protein Kinases/genetics,metabolism Protein-Tyrosine Kinases Viral Proteins/genetics,metabolism
Chemicals
Viral Proteins Protein Kinases Protein-Tyrosine Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Clinton G M
Roskoski R
References (19)
19 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.5.973-977.1984
Published
1984-05-00
Pages
973-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368851
Subset
IM
Grants
NCI NIH HHS · CA-34517 · United States
NHLBI NIH HHS · HL 24791 · United States
NINDS NIH HHS · NS-15997 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.5.973-977.1984 PMC Full Text View journal details
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