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PMID: 12869709 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Decreased peptidyltransferase activity correlates with increased programmed -1 ribosomal frameshifting and viral maintenance defects in the yeast Saccharomyces cerevisiae.

RNA (New York, N.Y.) ·Vol. 9 ·No. 8 ·2003-08-00 ·Pages 982-92

Meskauskas A, Harger JW, Jacobs KL, Dinman JD

Abstract

Increased efficiencies of programmed -1 ribosomal frameshifting in yeast cells expressing mutant forms of ribosomal protein L3 are unable to maintain the dsRNA "Killer" virus. Here we demonstrate that changes in frameshifting and virus maintenance in these mutants correlates with decreased peptidyltransferase activities. The mutants did not affect Ty1-directed programmed +1 ribosomal frameshifting or nonsense-mediated mRNA decay. Independent experiments demonstrate similar programmed -1 ribosomal frameshifting specific defects in cells lacking ribosomal protein L41, which has previously been shown to result in peptidyltransferase defects in yeast. These findings are consistent with the hypothesis that decreased peptidyltransferase activity should result in longer ribosome pause times after the accommodation step of the elongation cycle, allowing more time for ribosomal slippage at programmed -1 ribosomal frameshift signals.

MeSH Terms
Alleles Amino Acid Sequence Base Sequence DNA, Fungal Frameshifting, Ribosomal Molecular Sequence Data Peptidyl Transferases/metabolism Plasmids RNA, Messenger/genetics Saccharomyces cerevisiae/enzymology,genetics,virology Sequence Homology, Amino Acid
Chemicals
DNA, Fungal RNA, Messenger Peptidyl Transferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Meskauskas Arturas
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742, USA.
Harger Jason W
Jacobs Kristi L Muldoon
Dinman Jonathan D
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1261/rna.2165803
Published
2003-08-00
Pages
982-92
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1240118
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058859 · United States
NIGMS NIH HHS · R01 GM58859 · United States
External Links
PubMed source DOI: 10.1261/rna.2165803 PMC Full Text View journal details
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