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PMID: 10648594 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Kinetics of ribosomal pausing during programmed -1 translational frameshifting.

Molecular and cellular biology ·Vol. 20 ·No. 4 ·2000-02-00 ·Pages 1095-103

Lopinski JD, Dinman JD, Bruenn JA

Abstract

In the Saccharomyces cerevisiae double-stranded RNA virus, programmed -1 ribosomal frameshifting is responsible for translation of the second open reading frame of the essential viral RNA. A typical slippery site and downstream pseudoknot are necessary for this frameshifting event, and previous work has demonstrated that ribosomes pause over the slippery site. The translational intermediate associated with a ribosome paused at this position is detected, and, using in vitro translation and quantitative heelprinting, the rates of synthesis, the ribosomal pause time, the proportion of ribosomes paused at the slippery site, and the fraction of paused ribosomes that frameshift are estimated. About 10% of ribosomes pause at the slippery site in vitro, and some 60% of these continue in the -1 frame. Ribosomes that continue in the -1 frame pause about 10 times longer than it takes to complete a peptide bond in vitro. Altering the rate of translational initiation alters the rate of frameshifting in vivo. Our in vitro and in vivo experiments can best be interpreted to mean that there are three methods by which ribosomes pass the frameshift site, only one of which results in frameshifting.

MeSH Terms
Base Sequence Binding Sites Frameshifting, Ribosomal Kinetics Models, Genetic Nucleic Acid Conformation RNA, Viral/chemistry,genetics,metabolism Ribosomes/genetics,metabolism Saccharomyces cerevisiae/virology Totiviridae/genetics,metabolism
Chemicals
RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lopinski J D
Department of Biological Sciences, State University of New York at Buffalo, Buffalo, New York 14260, USA.
Dinman J D
Bruenn J A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.20.4.1095-1103.2000
Published
2000-02-00
Pages
1095-103
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC85227
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058859 · United States
NIGMS NIH HHS · GM22200 · United States
NIGMS NIH HHS · GM58859 · United States
External Links
PubMed source DOI: 10.1128/MCB.20.4.1095-1103.2000 PMC Full Text View journal details
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