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PMID: 3428275 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An efficient ribosomal frame-shifting signal in the polymerase-encoding region of the coronavirus IBV.

The EMBO journal ·Vol. 6 ·No. 12 ·1987-12-01 ·Pages 3779-85

Brierley I, Boursnell ME, Binns MM, Bilimoria B, Blok VC, Brown TD, Inglis SC

Abstract

The polymerase-encoding region of the genomic RNA of the coronavirus infectious bronchitis virus (IBV) contains two very large, briefly overlapping open reading frames (ORF), F1 and F2, and it has been suggested on the basis of sequence analysis that expression of the downstream ORF, F2, might be mediated through ribosomal frame-shifting. To examine this possibility a cDNA fragment containing the F1/F2 overlap region was cloned within a marker gene and placed under the control of the bacteriophage SP6 promoter in a recombinant plasmid. Messenger RNA transcribed from this plasmid, when translated in cell-free systems, specified the synthesis of polypeptides whose size was entirely consistent with the products predicted by an efficient ribosomal frame-shifting event within the overlap region. The nature of the products was confirmed by their reactivity with antisera raised against defined portions of the flanking marker gene. This is the first non-retroviral example of ribosomal frame-shifting in higher eukaryotes.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Coronaviridae/enzymology,genetics DNA-Directed RNA Polymerases/genetics Genes Genes, Viral Genetic Vectors Mice Molecular Sequence Data Nucleic Acid Conformation Promoter Regions, Genetic Protein Biosynthesis RNA, Messenger/genetics RNA, Viral/genetics Ribosomes/metabolism Transcription, Genetic
Chemicals
RNA, Messenger RNA, Viral DNA-Directed RNA Polymerases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Brierley I
Department of Pathology, University of Cambridge, UK.
Boursnell M E
Binns M M
Bilimoria B
Blok V C
Brown T D
Inglis S C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-12-01
Pages
3779-85
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553849
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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