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PMID: 1731118 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Ribosomal frameshifting requires a pseudoknot in the Saccharomyces cerevisiae double-stranded RNA virus.

Journal of virology ·Vol. 66 ·No. 2 ·1992-02-00 ·Pages 999-1006

Tzeng TH, Tu CL, Bruenn JA

Abstract

The large double-stranded RNA of the Saccharomyces cerevisiae (yeast) virus has two large overlapping open reading frames on the plus strand, one of which is translated via a -1 ribosomal frameshift. Sequences including the overlapping region, placed in novel contexts, can direct ribosomes to make a -1 frameshift in wheat germ extract, Escherichia coli and S. cerevisiae. This sequence includes a consensus slippery sequence, GGGUUUA, and has the potential to form a pseudoknot 3' to the putative frameshift site. Based on deletion analysis, a region of 71 nucleotides including the potential pseudoknot and the putative slippery sequence is sufficient for frameshifting. Site-directed mutagenesis demonstrates that the pseudoknot is essential for frameshifting.

MeSH Terms
Base Composition Base Sequence Chromosome Deletion Frameshift Mutation Models, Structural Molecular Sequence Data Mutagenesis, Site-Directed Nucleic Acid Conformation Oligodeoxyribonucleotides Open Reading Frames Plasmids Promoter Regions, Genetic RNA Viruses/genetics Restriction Mapping Ribosomes/metabolism Saccharomyces cerevisiae/genetics,physiology beta-Galactosidase/genetics,metabolism
Chemicals
Oligodeoxyribonucleotides beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tzeng T H
Department of Biological Sciences, State University of New York, Buffalo 14260.
Tu C L
Bruenn J A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.2.999-1006.1992
Published
1992-02-00
Pages
999-1006
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240802
Subset
IM
Grants
NIGMS NIH HHS · GM22200 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.2.999-1006.1992 PMC Full Text View journal details
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