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PMID: 1986362 Published · ppublish English Journal Article

A -1 ribosomal frameshift in a double-stranded RNA virus of yeast forms a gag-pol fusion protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 1 ·1991-01-01 ·Pages 174-8

Dinman JD, Icho T, Wickner RB

Abstract

The L-A double-stranded RNA (dsRNA) virus of Saccharomyces cerevisiae has two open reading frames (ORFs). ORF1 encodes the 80-kDa major coat protein (gag). ORF2, which is expressed only as a 180-kDa fusion protein with ORF1, encodes a single-stranded RNA-binding domain and has the consensus sequence for RNA-dependent RNA polymerases of (+)-strand and double-stranded RNA viruses (pol). We show that the 180-kDa protein is formed by -1 ribosomal frame-shifting by a mechanism indistinguishable from that of retro-viruses. Analysis of the "slippery site" suggests that a low probability of unpairing of the aminoacyl-tRNA from the 0-frame codon at the ribosomal A site reduces the efficiency of frameshifting more than the reluctance of a given tRNA to have its wobble base mispaired. Frameshifting of L-A requires a pseudoknot structure just downstream of the shift site. The efficiency of the L-A frameshift site is 1.8%, similar to the observed molar ratio in viral particles of the 180-kDa fusion protein to the major coat protein.

MeSH Terms
Amino Acid Sequence Base Sequence Frameshift Mutation Fusion Proteins, gag-pol/genetics Genetic Vectors Molecular Sequence Data Mutagenesis, Site-Directed Oligonucleotide Probes Open Reading Frames Plasmids Protein Biosynthesis RNA Viruses/genetics RNA, Double-Stranded/genetics Ribosomes/metabolism Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Fusion Proteins, gag-pol Oligonucleotide Probes RNA, Double-Stranded
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dinman J D
Section on the Genetics of Simple Eukaryotes, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
Icho T
Wickner R B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.1.174
Published
1991-01-01
Pages
174-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50772
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.1.174 PMC Full Text View journal details
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