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PMID: 2720781 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of an efficient coronavirus ribosomal frameshifting signal: requirement for an RNA pseudoknot.

Cell ·Vol. 57 ·No. 4 ·1989-05-19 ·Pages 537-47

Brierley I, Digard P, Inglis SC

Abstract

The genomic RNA of the coronavirus IBV contains an efficient ribosomal frameshifting signal at the junction of two overlapping open reading frames. We have defined by deletion analysis an 86 nucleotide sequence encompassing the overlap region which is sufficient to allow frameshifting in a heterologous context. The upstream boundary of the signal consists of the sequence UUUAAAC, which is the likely site of ribosomal slippage. We show by creation of complementary nucleotide changes that the RNA downstream of this "slippery" sequence folds into a tertiary structure termed a pseudoknot, the formation of which is essential for efficient frameshifting.

MeSH Terms
Base Composition Base Sequence Coronaviridae/genetics Genes, Regulator Molecular Sequence Data Molecular Structure RNA, Ribosomal/analysis,genetics,ultrastructure RNA, Viral/analysis,ultrastructure Signal Transduction
Chemicals
RNA, Ribosomal RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brierley I
Department of Pathology, University of Cambridge, England.
Digard P
Inglis S C
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(89)90124-4
Published
1989-05-19
Pages
537-47
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC7133225
Subset
IM
External Links
PubMed source DOI: 10.1016/0092-8674(89)90124-4 PMC Full Text View journal details
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