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PMID: 2429262 Published · ppublish English Journal Article

Does 5S RNA from E. coli have a pseudoknotted structure?

Nucleic acids research ·Vol. 14 ·No. 18 ·1986-09-25 ·Pages 7473-85

Göringer HU, Wagner R

Abstract

Chemical modification and limited enzymatic hydrolysis on isolated E. coli 5S RNA have provided informations on the secondary- and tertiary structure compatible with pseudoknotted structures for the A- and B-conformers of the molecule. Changes in the accessibility and reactivity of nucleotides in loop C and at the stem of helix IV in two different 5S RNA conformers are highly suggestive for interactions between bases C35 to C37 with G105 to G107 for the A-form and C38 to U40 and A94 to G96 with additional interactions of C35, C37 with G98 and G100 for the B-form. In both cases the molecules are folded forming pseudoknots and two quasi--continuous double stranded helices with coaxial stacking. The two structures are in perfect agreement with the biochemical data concerning the stability of the molecule and the chemical reactivities of individual nucleotides of the 5S RNA A- and B-conformers.

MeSH Terms
Base Sequence Endonucleases Escherichia coli/analysis Indicators and Reagents Models, Molecular Molecular Weight Nucleic Acid Conformation RNA, Bacterial/isolation & purification RNA, Ribosomal/isolation & purification Ribonuclease T1 Single-Strand Specific DNA and RNA Endonucleases
Chemicals
Indicators and Reagents RNA, Bacterial RNA, Ribosomal Endonucleases Ribonuclease T1 Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Göringer H U
Wagner R
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31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.18.7473
Published
1986-09-25
Pages
7473-85
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311763
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.18.7473 PMC Full Text View journal details
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