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PMID: 388350 Published · ppublish English Comparative Study Journal Article

Determination of base pairing in Escherichia coli and Bacillus stearothermophilus 5S RNAs by infrared spectroscopy.

Nucleic acids research ·Vol. 7 ·No. 4 ·1979-10-25 ·Pages 1043-57

Appel B, Erdmann VA, Stulz J, Ackerman T

Abstract

The extent of base pairing in Escherichia coli and Bacillus stearothermophilus 5S RNAs was determined by infrared spectroscopy. From the infrared spectra taken at 20 degrees and 52 degrees C it is concluded that E. coli and B. stearothermophlius 5S RNAs possess a large number of base pairs (Table I). Comparison of our results with those previously published using other methods leads to the conclusion that the structures of prokaryotic 5S RNAs involve a large number of tertiary interactions, in which the base pairing is not necessarily solely of the Watson-Crick type.

MeSH Terms
Base Composition Escherichia coli/analysis Geobacillus stearothermophilus/analysis Molecular Weight Nucleic Acid Conformation RNA, Bacterial Species Specificity Spectrophotometry, Infrared
Chemicals
RNA, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Appel B
Erdmann V A
Stulz J
Ackerman T
References (28)
28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.4.1043
Published
1979-10-25
Pages
1043-57
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342281
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.4.1043 PMC Full Text View journal details
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