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PMID: 1185796 Published · ppublish English Journal Article

The architecture of 5S rRNA and its relation to function.

Journal of molecular evolution ·Vol. 6 ·No. 1 ·1975-10-03 ·Pages 61-76

Fox GE, Woese CR

Abstract

An extensive comparative analysis of the available primary sequence data on 5S rRNA has been made. A universal secondary structure is presented for procaryotic 5S rRNA which contains four helical regions. Eucaryotic 5S rRNAs are found to have only three of these helices and thus have a somewhat different architecture. In addition, a highly conserved segment of more than thirty nucleotides is identified in the 5' half of the procaryotic molecule. This segment includes the oligonucleotide -CGAAC- which presumably binds to the t-RNA "common" sequence -GTpsiCG-. Among the eucaryotes, the plants display a procaryotic nature in this region, but no eucaryote has the sequence -CGAAC- in this segment. A functional role for the procaryotic 5S rRNA molecule is discussed in which it is envisioned to undergo conformational change, i.e., coiling and uncoiling of one of the helices, which can result in a cyclic interaction of the 5S rRNA molecule with two t-RNA molecules. A general principle also emerges: the natural rotational motion inherent in coiling and uncoiling of nucleic acid helices can be converted quite simply to linear mechanical motion.

MeSH Terms
Base Sequence Biological Evolution Nucleic Acid Conformation Phylogeny RNA, Ribosomal/metabolism RNA, Transfer/metabolism Structure-Activity Relationship
Chemicals
RNA, Ribosomal RNA, Transfer
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fox G E
Woese C R
References (49)
49 references, click to expand
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Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/BF01732674
Published
1975-10-03
Pages
61-76
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01732674 View journal details
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