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PMID: 4355369 Published · ppublish English Journal Article

ATPase and GTPase activities associated with a specific 5S RNA-protein complex.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 10 ·1973-10-00 ·Pages 2870-3

Horne JR, Erdmann VA

Abstract

An RNA-protein complex consisting of 5S RNA and two ribosomal proteins, B-L5 and B-L22, was isolated from Bacillus stearothermophilus ribosomes and found to be active in GTP hydrolysis. This activity was not influenced by elongation factor G. Further analysis of this complex showed that it was also able to hydrolyze ATP. Inhibition studies revealed that ATP was a noncompetitive inhibitor of GTP and that GTP was also a noncompetitive inhibitor of ATP, indicating that two different enzymatic sites were involved. Differences in pH optimum and optimal temperature also point to a twosite enzyme complex. Both enzymatic hydrolyses were inhibited by thiostrepton and fusidic acid, which are known inhibitors of protein synthesis.

MeSH Terms
Adenosine Triphosphatases/antagonists & inhibitors,metabolism Adenosine Triphosphate/pharmacology Anti-Bacterial Agents/pharmacology Bacillus/enzymology Bacterial Proteins/metabolism Fusidic Acid/pharmacology Guanosine Triphosphate/pharmacology Hydrogen-Ion Concentration Hydrolysis Kinetics Peptides/pharmacology Phosphoric Monoester Hydrolases/antagonists & inhibitors,metabolism Phosphorus Radioisotopes Protein Binding RNA, Bacterial/metabolism RNA, Ribosomal/metabolism Temperature
Chemicals
Anti-Bacterial Agents Bacterial Proteins Peptides Phosphorus Radioisotopes RNA, Bacterial RNA, Ribosomal Fusidic Acid Guanosine Triphosphate Adenosine Triphosphate Phosphoric Monoester Hydrolases Adenosine Triphosphatases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Horne J R
Erdmann V A
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.10.2870
Published
1973-10-00
Pages
2870-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427128
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.10.2870 PMC Full Text View journal details
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