Two distinct conformations of rat liver ribosomal 5S RNA.

Nucleic acids research ·Vol. 10 ·No. 11 ·1982-06-11 ·Pages 3381-9

Toots I, Misselwitz R, Böhm S, Welfle H, Villems R, Saarma M

Abstract

Three different conformers of rat liver 5S ribosomal RNA were investigated by partial nuclease cleavage technique using S1 nuclease and cobra venom endoribonuclease (CVE) as conformational probes. Urea-treated and renatured 5S RNA co-migrate on non-denaturing gels, but exhibit distinct differences in their nuclease cleavage patterns. The most prominent differences in S1 nuclease and CVE accessibility of these conformers are located in region 30-50 and around nucleotides 70 and 90. The third form of 5S RNA with higher electrophoretic mobility was generated by EDTA treatment. The cleavage patterns of this 5S RNA conformer are similar to that characteristic for the renatured 5S RNA. The results demonstrate the difference in secondary structure and possibly different tertiary base-pairing interactions of 5S RNA conformers.

MeSH Terms

Animals Base Sequence Liver/analysis Molecular Weight Nucleic Acid Conformation Oligoribonucleotides/analysis RNA, Ribosomal Rats Ribosomes/analysis

Chemicals

Oligoribonucleotides RNA, Ribosomal

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Toots I

Misselwitz R

Böhm S

Welfle H

Villems R

Saarma M

References (27)

27 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.11.3381

Published

1982-06-11

Pages

3381-9

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320718

Subset

External Links

PubMed source DOI: 10.1093/nar/10.11.3381 PMC Full Text View journal details

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Two distinct conformations of rat liver ribosomal 5S RNA.

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