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PMID: 6272219 Published · ppublish English Journal Article

Location of single-stranded and double-stranded regions in rat liver ribosomal 5S RNA and 5.8S RNA.

Nucleic acids research ·Vol. 9 ·No. 20 ·1981-10-24 ·Pages 5331-43

Toots I, Metspalu A, Villems R, Saarma M

Abstract

Rat liver 5S rRNA and 5.8S rRNA were end-labelled with 32P at 5'-end or 3'-end of the polynucleotide chain and partially digested with single-strand specific S1 nuclease and double-strand specific endonuclease from the cobra Naja naja oxiana venom. The parallel use of these two structure-specific enzymes in combination with rapid sequencing technique allowed the exact localization of single-stranded and double-stranded regions in 5S RNA and 5.8 S RNA. The most accessible regions to S1 nuclease in 5S RNA are regions 33-42, 74-78, 102-103 and in 5.8 S RNA 16-20, 26-29, 34-36, 74-80 and a region around 125-130. The cobra venom endonuclease cleaves the following areas in 5S RNA: 7-8, 17-20, 28-30, 49-51, 56-57, 60-64, 69-70, 81-82, 95-97, 106-112. In 5.8S RNA the venom endonuclease cleavage sites are 4-7, 10-13, 21-22, 33-35, 43-45, 51-55, 72-74, 85-87, 98-99, 105-106, 114-115, 132-135. According to these results the tRNA binding sequences proposed by Nishikawa and Takemura [(1974) FEBS Lett. 40, 106-109], in 5S RNA are located in partly single-stranded region, but in 5.8S RNA in double-stranded region.

MeSH Terms
Animals Base Sequence Endonucleases Liver/analysis Nucleic Acid Conformation RNA, Double-Stranded/analysis RNA, Ribosomal/analysis Rats Ribonucleases Ribosomes/analysis
Chemicals
RNA, Double-Stranded RNA, Ribosomal Endonucleases Ribonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Toots I
Metspalu A
Villems R
Saarma M
References (34)
34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.20.5331
Published
1981-10-24
Pages
5331-43
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327523
Subset
IM
Databases
GENBANK
J01881, K01594
External Links
PubMed source DOI: 10.1093/nar/9.20.5331 PMC Full Text View journal details
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