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PMID: 7041089 Published · ppublish English Journal Article

A structural model of 5S RNA from E. coli based on intramolecular crosslinking evidence.

Nucleic acids research ·Vol. 10 ·No. 4 ·1982-02-25 ·Pages 1257-69

Hancock J, Wagner R

Abstract

We describe new results obtained using the bifunctional chemical reagent phenyldiglyoxal (PDG) to study the intramolecular crosslinking of ribosomal 5S RNA from E. coli. In a previous publication (Wagner & Garrett [1]) we reported the identification of a crosslink in the stem region of 5S RNA (G2-G112) using the same reagent but were unable to obtain further information because of the presence of monofunctional adducts which confused the analyses. To overcome this problem, we have removed the monoaddition products by coupling them via their free reagent ends to a solid support bearing reactive groups. Using this system we have been able to identify a new crosslink G41-G72 in native 5S RNA which has considerable structural implications. We propose a structural model in which the proximity of both nucleotides is maintained by secondary interactions.

MeSH Terms
Base Sequence Escherichia coli/analysis Nucleic Acid Conformation Oligoribonucleotides/analysis RNA, Ribosomal/isolation & purification
Chemicals
Oligoribonucleotides RNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hancock J
Wagner R
References (22)
22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.4.1257
Published
1982-02-25
Pages
1257-69
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320523
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.4.1257 PMC Full Text View journal details
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