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PMID: 6368221 Published · ppublish English Journal Article

Cloning and sequencing of the preprotoxin-coding region of the yeast M1 double-stranded RNA.

The EMBO journal ·Vol. 3 ·No. 1 ·1984-01-00 ·Pages 107-11

Skipper N, Thomas DY, Lau PC

Abstract

Complementary DNA (cDNA) copies of the M1-1, toxin-coding region of the yeast M1 double-stranded RNA (dsRNA) have been cloned and sequenced. These sequences, in combination with the known terminal sequence of M1-1 dsRNA, identify a translation reading frame for a 316 amino acid protein of 34.7 kd, similar in size to the preprotoxin produced from M1 dsRNA by in vitro translation. Potential glycosylation sites in the preprotoxin peptide are identified. Based on its methionine content the extracellular yeast toxin appears to be contained within the C-terminal region of the precursor.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/metabolism Mycotoxins/genetics Protein Biosynthesis Protein Precursors/genetics RNA, Double-Stranded/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Mycotoxins Protein Precursors RNA, Double-Stranded DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Skipper N
Thomas D Y
Lau P C
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35 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-01-00
Pages
107-11
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557305
Subset
IM
Databases
GENBANK
X00285
External Links
PubMed source PMC Full Text View journal details
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