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PMID: 9858562 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Ribosomal protein L3 mutants alter translational fidelity and promote rapid loss of the yeast killer virus.

Molecular and cellular biology ·Vol. 19 ·No. 1 ·1999-01-00 ·Pages 384-91

Peltz SW, Hammell AB, Cui Y, Yasenchak J, Puljanowski L, Dinman JD

Abstract

Programmed -1 ribosomal frameshifting is utilized by a number of RNA viruses as a means of ensuring the correct ratio of viral structural to enzymatic proteins available for viral particle assembly. Altering frameshifting efficiencies upsets this ratio, interfering with virus propagation. We have previously demonstrated that compounds that alter the kinetics of the peptidyl-transfer reaction affect programmed -1 ribosomal frameshift efficiencies and interfere with viral propagation in yeast. Here, the use of a genetic approach lends further support to the hypothesis that alterations affecting the ribosome's peptidyltransferase activity lead to changes in frameshifting efficiency and virus loss. Mutations in the RPL3 gene, which encodes a ribosomal protein located at the peptidyltransferase center, promote approximately three- to fourfold increases in programmed -1 ribosomal frameshift efficiencies and loss of the M1 killer virus of yeast. The mak8-1 allele of RPL3 contains two adjacent missense mutations which are predicted to structurally alter the Mak8-1p. Furthermore, a second allele that encodes the N-terminal 100 amino acids of L3 (called L3Delta) exerts a trans-dominant effect on programmed -1 ribosomal frameshifting and killer virus maintenance. Taken together, these results support the hypothesis that alterations in the peptidyltransferase center affect programmed -1 ribosomal frameshifting.

MeSH Terms
Alleles Exoribonucleases/genetics Exosome Multienzyme Ribonuclease Complex Frameshift Mutation Gene Expression Mutation Peptidyl Transferases/antagonists & inhibitors Plasmids RNA Viruses/genetics Ribosomal Protein L3 Ribosomal Proteins/genetics Saccharomyces cerevisiae/virology Saccharomyces cerevisiae Proteins Transcription, Genetic
Chemicals
Ribosomal Protein L3 Ribosomal Proteins SKI6 protein, S cerevisiae Saccharomyces cerevisiae Proteins Peptidyl Transferases Exoribonucleases Exosome Multienzyme Ribonuclease Complex
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Peltz S W
The Cancer Institute of New Jersey, Piscataway, New Jersey 08854, USA.
Hammell A B
Cui Y
Yasenchak J
Puljanowski L
Dinman J D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.19.1.384
Published
1999-01-00
Pages
384-91
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC83896
Subset
IM
Grants
NIGMS NIH HHS · R01 GM048631 · United States
NIGMS NIH HHS · R01 GM058859 · United States
NIAID NIH HHS · T32 AI07403-07 · United States
NIGMS NIH HHS · GM48631 · United States
NIAID NIH HHS · T32 AI007403 · United States
External Links
PubMed source DOI: 10.1128/MCB.19.1.384 PMC Full Text View journal details
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