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PMID: 8138178 Published · ppublish English Journal Article

Translational maintenance of frame: mutants of Saccharomyces cerevisiae with altered -1 ribosomal frameshifting efficiencies.

Genetics ·Vol. 136 ·No. 1 ·1994-01-00 ·Pages 75-86

Dinman JD, Wickner RB

Abstract

A special site on the (+) strand of the L-A dsRNA virus induces about 2% of ribosomes translating the gag open reading frame to execute a -1 frameshift and thus produce the viral gag-pol fusion protein. Using constructs in which a -1 ribosomal frameshift at this site was necessary for expression of lacZ we isolated chromosomal mutants in which the efficiency of frameshifting was increased. These mutants comprise eight genes, named mof (maintenance of frame). The mof1-1, mof2-1, mof4-1, mof5-1 and mof6-1 strains cannot maintain M1 dsRNA at 30 degrees, but, paradoxically, do not lose L-A. The mof2-1, mof5-1 and mof6-1 strains are temperature sensitive for growth at 37 degrees, and all three show striking cell cycle phenotypes. The mof2-1 strains arrest with mother and daughter cells almost equal in size, mof5-1 arrests with multiple buds and mof6-1 arrests as single large unbudded cells. mof2-1 and mof5-1 strains are also Pet-. The mof mutations show differential effects on various frameshifting signals.

Related Genes
gag lacZ mof mof1-1 mof2-1 mof4-1 mof5-1 mof6-1
MeSH Terms
Base Sequence Escherichia coli/genetics Frameshift Mutation Genes, Fungal Genes, gag Genetic Complementation Test Genotype Meiosis Mutagenesis Open Reading Frames Phenotype Plasmids Protein Biosynthesis RNA Viruses/genetics,metabolism RNA, Double-Stranded/genetics,metabolism Ribosomes/metabolism Saccharomyces cerevisiae/genetics,metabolism beta-Galactosidase/biosynthesis,metabolism
Chemicals
RNA, Double-Stranded beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dinman J D
Laboratory of Biochemical Pharmacology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Wickner R B
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/136.1.75
Published
1994-01-00
Pages
75-86
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205794
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/136.1.75 PMC Full Text View journal details
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