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PMID: 8413200 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Pi, a pre-B-cell-specific enhancer element in the immunoglobulin heavy-chain enhancer.

Molecular and cellular biology ·Vol. 13 ·No. 10 ·1993-10-00 ·Pages 5957-69

Libermann TA, Baltimore D

Abstract

We have identified a new immunoglobulin heavy-chain enhancer element, designated pi, between the microE2 and microE3 elements. The pi enhancer element is transcriptionally active primarily during early stages of B-cell development but becomes virtually inactive during B-cell maturation at about the stage of immunoglobulin kappa light-chain gene rearrangement. Mutational analysis suggests that the pi element is crucial for immunoglobulin heavy-chain enhancer activity at the pre-B-cell stage but is almost irrelevant for enhancer activity at the mature B-cell or plasma-cell stage. The activity of the pi enhancer element correlates with the presence of an apparently pre-B-cell-specific protein-DNA complex. The similarity of the pi site to recognition sequences for members of the ets gene family suggests that the protein(s) interacting with the pi site most likely are ets-related transcription factors.

Related Genes
ets
MeSH Terms
3T3 Cells Animals B-Lymphocytes Base Sequence Binding Sites Biological Evolution Cell Line Cloning, Molecular DNA Enhancer Elements, Genetic HeLa Cells Humans Immunoglobulin Heavy Chains/genetics Mice Molecular Sequence Data Multigene Family Mutation Restriction Mapping Retroviridae Proteins, Oncogenic/genetics Transcription Factors/metabolism Tumor Cells, Cultured
Chemicals
Immunoglobulin Heavy Chains Retroviridae Proteins, Oncogenic Transcription Factors oncogene proteins v-ets DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Libermann T A
Department of Medicine, Beth Israel Hospital, Boston, Massachusetts 02215.
Baltimore D
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71 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.10.5957-5969.1993
Published
1993-10-00
Pages
5957-69
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364640
Subset
IM
Grants
NIGMS NIH HHS · GM 39458 · United States
External Links
PubMed source DOI: 10.1128/mcb.13.10.5957-5969.1993 PMC Full Text View journal details
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