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PMID: 2180582 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene.

Cell ·Vol. 61 ·No. 1 ·1990-04-06 ·Pages 113-24

Klemsz MJ, McKercher SR, Celada A, Van Beveren C, Maki RA

Abstract

We have isolated a cDNA clone, PU.1, that codes for a new tissue-specific DNA binding protein. Analysis of the binding site by methylation interference and DNAase 1 protection revealed that the PU.1 protein recognized a purine-rich sequence, 5'-GAGGAA-3' (PU box). The PU.1 protein was shown to be a transcriptional activator that is expressed in macrophages and B cells. cDNA constructions used to generate proteins lacking portions of either the amino- or carboxy-terminal ends of the PU.1 protein placed the DNA binding domain in the highly basic carboxy-terminal domain of the protein. The amino acid sequence in the binding domain of PU.1 has considerable identity with proteins belonging to the ets oncogene family.

MeSH Terms

Amino Acid Sequence Animals B-Lymphocytes/metabolism Base Sequence Cell Nucleus/metabolism Cloning, Molecular DNA/genetics,isolation & purification DNA-Binding Proteins/genetics Gene Expression Regulation Gene Library Genes Immunoblotting Macrophages/metabolism Methylation Mice Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotide Probes Protein Biosynthesis Protein Conformation Protein-Tyrosine Kinases/genetics Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-ets Proto-Oncogenes Restriction Mapping Retroviridae Proteins, Oncogenic Sequence Homology, Nucleic Acid Transcription Factors/genetics Transcription, Genetic

Chemicals

DNA-Binding Proteins Oligonucleotide Probes Proto-Oncogene Proteins Proto-Oncogene Proteins c-ets Retroviridae Proteins, Oncogenic Transcription Factors v-Spi-1 protein, Friend spleen focus-forming virus DNA Protein-Tyrosine Kinases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Klemsz M J

Cancer Research Center, La Jolla Cancer Research Foundation, California 92037.

McKercher S R

Celada A

Van Beveren C

Maki R A

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/0092-8674(90)90219-5

Published

1990-04-06

Pages

113-24

Language

English

Region

United States

NLM ID

0413066

Subset

Grants

NIAID NIH HHS · AI20194 · United States

Databases

GENBANK

M32370

Corrections

CommentIn

PMID: 2364426

External Links

PubMed source DOI: 10.1016/0092-8674(90)90219-5 View journal details

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The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene.

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