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PMID: 3031612 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nuclear factors binding to the human immunoglobulin heavy-chain gene enhancer.

Nucleic acids research ·Vol. 15 ·No. 7 ·1987-04-10 ·Pages 2851-69

Maeda H, Araki K, Kitamura D, Wang J, Watanabe T

Abstract

The human immunoglobulin heavy chain (IgH) gene contains at least two tissue-specific regulatory regions, which are similar to the mouse IgH gene. One is the J-C enhancer and another is located in the 5' promoter region. Using an electrophoretic mobility shift assay and DNase I footprint, we have examined the interaction of factors in B cell nuclear extracts with the two regulatory regions of the human IgH gene. We have identified a nuclear factor in mouse B cell nuclear extracts which bound to specific sequence in the human IgH enhancer. This factor is apparently not present in mouse fibroblast nuclear extracts. We also found factor(s) which bound to the highly conserved octanucleotide sequence within the human IgH enhancer and 5' promoter regions.

MeSH Terms
Animals B-Lymphocytes/immunology Base Sequence Cell Line Cell Nucleus/metabolism DNA Restriction Enzymes Enhancer Elements, Genetic Genes Genes, Regulator Humans Immunoglobulin Heavy Chains/genetics Introns Mice Plasmacytoma Plasmids
Chemicals
Immunoglobulin Heavy Chains DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Maeda H
Araki K
Kitamura D
Wang J
Watanabe T
References (43)
43 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.7.2851
Published
1987-04-10
Pages
2851-69
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340703
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.7.2851 PMC Full Text View journal details
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