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PMID: 3095795 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Functional analysis of the murine IgH enhancer: evidence for negative control of cell-type specificity.

Nucleic acids research ·Vol. 14 ·No. 20 ·1986-10-24 ·Pages 8209-21

Kadesch T, Zervos P, Ruezinsky D

Abstract

We have carried out a mutational analysis of the mouse IgH enhancer. Consistent with previous reports, deletions extending from either the 5' side or the 3' side of the enhancer fail to reveal distinct boundaries which define enhancer function in lymphoid cells. Interestingly, internal point mutations and deletions within the "enhancer core" regions fail to identify any necessary functional role for these conserved elements. When tested in CV1 cells, which do not normally respond to the IgH enhancer, certain deletions exhibit significant enhancer activity. We take these findings to indicate that the functional domains of the IgH enhancer are complex and that cell type specificity is defined in part by negative factors present in non-lymphoid cells.

MeSH Terms
Animals Base Sequence Cell Line Chromosome Deletion Enhancer Elements, Genetic Gene Expression Regulation Genes, Regulator Genetic Engineering Haplorhini Immunoglobulin Heavy Chains/genetics Mice Structure-Activity Relationship Transcription, Genetic
Chemicals
Immunoglobulin Heavy Chains
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kadesch T
Zervos P
Ruezinsky D
References (34)
34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.20.8209
Published
1986-10-24
Pages
8209-21
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311845
Subset
IM
Grants
NIGMS NIH HHS · 2-T32-GM-07229-11 · United States
Databases
GENBANK
X04489
External Links
PubMed source DOI: 10.1093/nar/14.20.8209 PMC Full Text View journal details
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