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PMID: 1901991 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

An enhancer at the 3' end of the mouse immunoglobulin heavy chain locus.

Nucleic acids research ·Vol. 19 ·No. 4 ·1991-02-25 ·Pages 933-7

Lieberson R, Giannini SL, Birshtein BK, Eckhardt LA

Abstract

A tissue-specific enhancer (E mu) lies between the joining (JH) and mu constant region (C mu) gene segments of the immunoglobulin heavy chain (IgH) locus. Since mouse endogenous IgH genes are efficiently transcribed in its absence, the normal function of this enhancer remains ill-defined. Recently, another lymphoid-specific enhancer of equal strength has been identified 3' of the rat IgH locus. We have isolated an analogous sequence from mouse and have mapped it 12.5 kb 3' of the 3'-most constant region gene (C alpha-membrane) of the BALB/c mouse locus. The mouse and rat sequences are 82% homologous and share with other enhancers several DNA sequence motifs capable of binding protein. However, in transient transfection assays, the mouse sequence behaves as a weaker enhancer. The role of this distant element in the expression of endogenous IgH genes, both in E mu-deficient, Ig-producing cell lines and during normal B cell development, is discussed.

MeSH Terms
Animals Chloramphenicol O-Acetyltransferase/genetics Chromosome Mapping Cloning, Molecular DNA/analysis Electrophoresis, Agar Gel Enhancer Elements, Genetic Immunoglobulin Heavy Chains/genetics Mice Mice, Inbred BALB C Molecular Sequence Data Plasmids Rats Sequence Homology, Nucleic Acid Transcription, Genetic Transfection
Chemicals
Immunoglobulin Heavy Chains DNA Chloramphenicol O-Acetyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lieberson R
Department of Biological Sciences, Columbia University, New York, NY 10027.
Giannini S L
Birshtein B K
Eckhardt L A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.4.933
Published
1991-02-25
Pages
933-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333734
Subset
IM
Grants
NIAID NIH HHS · AI10702 · United States
NIAID NIH HHS · AI13509 · United States
NIAID NIH HHS · AI30653 · United States
External Links
PubMed source DOI: 10.1093/nar/19.4.933 PMC Full Text View journal details
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