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PMID: 6171827 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

RNA splicing mutation in an aberrantly rearranged immunoglobulin lambda I gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 11 ·1981-11-00 ·Pages 7019-23

Hozumi N, Wu GE, Murialdo H, Roberts L, Vetter D, Fife WL, Whiteley M, Sadowski P

Abstract

The mouse cell line MOPC 315 is an IgA (lambda II)-producing myeloma. We have studied a derivative of MOPC 315 that secretes normal lambda II chains but no heavy chain. This derivative, MOPC 315-26, was found to contain a rearranged lambda I gene in addition to a rearranged lambda II gene. The rearranged lambda I gene was cloned into bacteriophage lambda DNA and its structure was studied. The lambda I gene was found to have arisen by an aberrant recombination event that resulted in a single base insertion at the site of V-J region joining. In addition, the gene contained numerous point mutations in the vicinity of the junction of the V and J regions. Two point mutations occurred in the donor splice sequence normally used for the removal of the intron between the J and C regions, suggesting that the RNA synthesized from the aberrantly rearranged lambda I gene would be unable to undergo proper RNA splicing.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics Genes Immunoglobulin A/genetics Mice Mutation Plasmacytoma RNA/genetics Recombination, Genetic
Chemicals
Immunoglobulin A RNA DNA Restriction Enzymes
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Hozumi N
Wu G E
Murialdo H
Roberts L
Vetter D
Fife W L
Whiteley M
Sadowski P
References (38)
38 references, click to expand
  1. Cellular localization of immunoglobulins with different allotypic specificities in rabbit lymphoid tissues.
    J Exp Med. 1965 Nov 1;122(5):853-76 PMID: 4159057
  2. Activity of multiple light chain genes in murine myeloma cells producing a single, functional light chain.
    Cell. 1980 Aug;21(1):1-12 PMID: 6773666
  3. Origin of antibody variation.
    Nature. 1966 Jul 16;211(5046):242-3 PMID: 5965537
  4. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.
    Virology. 1970 Mar;40(3):734-44 PMID: 4908735
  5. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  6. Isolation of high-molecular-weight DNA from mammalian cells.
    Eur J Biochem. 1973 Jul 2;36(1):32-8 PMID: 4200179
  7. Isolation of the bacteriophage lambda A-gene protein.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):581-5 PMID: 1054839
  8. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  9. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  10. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  11. Genetic control of antibody variable regions.
    Cold Spring Harb Symp Quant Biol. 1977;41 Pt 2:837-46 PMID: 408080
  12. Variable and constant parts of the immunoglobulin light chain gene of a mouse myeloma cell are 1250 nontranslated bases apart.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5652-6 PMID: 414223
  13. Modification and fate of J chain in myeloma cells in the presence and absence of polymeric immunoglobulin secretion.
    Eur J Immunol. 1978 Feb;8(2):94-101 PMID: 95957
  14. A complete immunoglobulin gene is created by somatic recombination.
    Cell. 1978 Sep;15(1):1-14 PMID: 100225
  15. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  16. Immunoglobulin gene rearrangement in immature B cells.
    Science. 1980 Sep 19;209(4463):1366-9 PMID: 6774416
  17. Functional and non-functional joining in immunoglobulin light chain genes of a mouse myeloma.
    Nature. 1980 Oct 16;287(5783):603-7 PMID: 6776411
  18. Variation in the crossover point of kappa immunoglobulin gene V-J recombination: evidence from a cryptic gene.
    Cell. 1980 Oct;21(3):793-9 PMID: 6777049
  19. Alternatives for splicing: recognizing the ends of introns.
    Cell. 1980 Nov;22(2 Pt 2):324-6 PMID: 6160915
  20. Dual expression of lambda genes in the MOPC-315 plasmacytoma.
    Nature. 1981 Mar 5;290(5801):65-7 PMID: 6259534
  21. Human immunoglobulin kappa light-chain genes are deleted or rearranged in lambda-producing B cells.
    Nature. 1981 Apr 2;290(5805):368-72 PMID: 6783958
  22. Aberrant rearrangements contribute significantly to the allelic exclusion of immunoglobulin gene expression.
    Nature. 1981 Apr 2;290(5805):372-8 PMID: 6783959
  23. IgG antibodies to phosphorylcholine exhibit more diversity than their IgM counterparts.
    Nature. 1981 May 7;291(5810):29-34 PMID: 7231520
  24. Misalignment of V and J gene segments resulting in a nonfunctional immunoglobulin gene.
    Nucleic Acids Res. 1981 Mar 11;9(5):1101-9 PMID: 6164985
  25. Molecular cloning of an immunoglobulin kappa constant gene from NZB mouse.
    Gene. 1981 Mar;13(2):163-172 PMID: 6453743
  26. Sequences of mouse immunoglobulin light chain genes before and after somatic changes.
    Cell. 1978 Dec;15(4):1133-44 PMID: 103630
  27. Sequences at the somatic recombination sites of immunoglobulin light-chain genes.
    Nature. 1979 Jul 26;280(5720):288-94 PMID: 111144
  28. A kappa-immunoglobulin gene is formed by site-specific recombination without further somatic mutation.
    Nature. 1979 Aug 2;280(5721):370-5 PMID: 111146
  29. Early intermediates in bacteriophage lambda prohead assembly.
    Virology. 1979 Jul 30;96(2):341-67 PMID: 380144
  30. Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3450-4 PMID: 115000
  31. Immunoglobulin production and secretion by variant clones of the MOPC 315 mouse myeloma cell line.
    J Immunol. 1980 Mar;124(3):1174-82 PMID: 6766968
  32. Organization of kappa light chain genes in germ-line and somatic tissue.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):1106-10 PMID: 6244580
  33. Recovery of DNA from gels.
    Methods Enzymol. 1980;65(1):371-80 PMID: 7374460
  34. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  35. Transcription of mouse kappa chain genes: implications for allelic exclusion.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1937-41 PMID: 6769117
  36. RNA splicing generates a variant light chain from an aberrantly rearranged kappa gene.
    Nature. 1980 Aug 21;286(5775):776-9 PMID: 6772972
  37. A mutant immunoglobulin light chain is formed by aberrant DNA- and RNA-splicing events.
    Nature. 1980 Aug 21;286(5775):779-83 PMID: 6772973
  38. Rabbit lymphoid cells differentiated with respect to alpha-, gamma-, and mu- heavy polypeptide chains and to allotypic markers Aa1 and Aa2.
    J Exp Med. 1966 Mar 1;123(3):547-58 PMID: 4160817
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.11.7019
Published
1981-11-00
Pages
7019-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349185
Subset
IM
Databases
GENBANK
J00580, J00581
External Links
PubMed source DOI: 10.1073/pnas.78.11.7019 PMC Full Text View journal details
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