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PMID: 414223 Published · ppublish English Journal Article

Variable and constant parts of the immunoglobulin light chain gene of a mouse myeloma cell are 1250 nontranslated bases apart.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 12 ·1977-12-00 ·Pages 5652-6

Brack C, Tonegawa S

Abstract

A DNA fragment carrying an immunoglobulin gene coding for both variable (V) and constant (C) regions of a mouse lambda light chain was enriched about 15-fold from a total endonuclease EcoRI digest of a plasmacytoma (HOPC 2020) DNA by preparative agarose gel electrophoresis. The DNA fraction was used for cloning of a lambda chain gene in the phage lambdagtWES vector. After screening [Benton, W. D. & Davis, R. W. (1977) Science 196, 180-182] of about 70,000 plaques, each arising from an independent transfection event, we isolated one clone (Ig 303) that contained both a Vlambda and a Clambda DNA sequence. Electron microscopy of R-loops formed between the cloned DNA and purified lambda chain mRNA (HOPC 2020) revealed that the Vlambda and Clambda DNA sequences are separated by a 1250-base DNA fragment.

MeSH Terms
Animals Base Sequence Binding Sites, Antibody DNA, Neoplasm/genetics Genes Immunoglobulin Light Chains/genetics Immunoglobulin Variable Region Immunoglobulin lambda-Chains/genetics Mice Myeloma Proteins/genetics Neoplasms, Experimental/genetics Nucleic Acid Hybridization Plasmacytoma/genetics Protein Biosynthesis RNA, Messenger/genetics
Chemicals
DNA, Neoplasm Immunoglobulin Light Chains Immunoglobulin Variable Region Immunoglobulin lambda-Chains Myeloma Proteins RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brack C
Tonegawa S
References (18)
18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.12.5652
Published
1977-12-00
Pages
5652-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431846
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.12.5652 PMC Full Text View journal details
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