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PMID: 2850479 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure of the murine lck gene and its rearrangement in a murine lymphoma cell line.

Molecular and cellular biology ·Vol. 8 ·No. 8 ·1988-08-00 ·Pages 3058-64

Garvin AM, Pawar S, Marth JD, Perlmutter RM

Abstract

The lck gene encodes a lymphocyte-specific protein-tyrosine kinase that is implicated in neoplastic transformation. We have determined the germ line organization of the murine lck gene and have isolated and characterized a rearranged lck allele in the murine lymphoma cell line LSTRA. The overall exon-intron organization of the normal lck gene is almost identical to that of avian c-src. In LSTRA DNA, an internally rearranged Moloney murine leukemia virus genome is interposed between two distinct promoters that normally generate lck transcripts differing only in 5' untranslated regions. The rearrangement appears to have been selected to permit splicing of transcripts that initiate from the Moloney virus promoter to an acceptor site located within the first exon 3' to the downstream promoter, thus generating an lck mRNA with a novel 5' untranslated region that may be more efficiently translated.

MeSH Terms
Animals Base Sequence Cell Line DNA Restriction Enzymes Genes Humans Lymphocytes/enzymology Lymphoma/enzymology,genetics Mice Molecular Sequence Data Protein-Tyrosine Kinases/genetics Sequence Homology, Nucleic Acid Species Specificity
Chemicals
Protein-Tyrosine Kinases DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Garvin A M
Howard Hughes Medical Institute, University of Washington, Seattle 98195.
Pawar S
Marth J D
Perlmutter R M
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36 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.8.3058-3064.1988
Published
1988-08-00
Pages
3058-64
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363532
Subset
IM
Grants
NIGMS NIH HHS · GM07270 · United States
Databases
GENBANK
M21510, M21511
External Links
PubMed source DOI: 10.1128/mcb.8.8.3058-3064.1988 PMC Full Text View journal details
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