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PMID: 6095054 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation, linkage, and sequence of mouse metallothionein I and II genes.

Molecular and cellular biology ·Vol. 4 ·No. 7 ·1984-07-00 ·Pages 1221-30

Searle PF, Davison BL, Stuart GW, Wilkie TM, Norstedt G, Palmiter RD

Abstract

The mouse metallothionein II (MT-II) gene is located approximately 6 kilobases upstream of the MT-I gene. A comparison of the sequences of mouse MT-I and MT-II genes (as well as those of other mammals) reveals that the coding regions are highly conserved even at "silent" positions but that the noncoding regions and introns are extremely divergent between primates and rodents. There are four blocks of conserved sequences in the promoters of mouse MT-I, mouse MT-II, and human MT-IIA genes; one includes the TATAAA sequence, and another has been implicated in regulation by heavy metals. Mouse MT-I and MT-II mRNAs are induced to approximately the same extent in vivo in response to cadmium, dexamethasone, or lipopolysaccharide. Mouse MT-I and MT-II genes are regulated by metals but not by glucocorticoids after transfection into HeLa cells.

MeSH Terms
Amino Acid Sequence Animals Bacteriophage lambda Base Sequence Cell Line Cell Nucleus/metabolism DNA Restriction Enzymes Genes Genes, Regulator Genetic Linkage Humans Metallothionein/genetics Mice Nucleic Acid Hybridization Species Specificity Transcription, Genetic/drug effects Zinc/pharmacology
Chemicals
Metallothionein DNA Restriction Enzymes Zinc
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Searle P F
Davison B L
Stuart G W
Wilkie T M
Norstedt G
Palmiter R D
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37 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.7.1221-1230.1984
Published
1984-07-00
Pages
1221-30
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368902
Subset
IM
Grants
NIGMS NIH HHS · GM-07270 · United States
NICHD NIH HHS · HD-09172 · United States
Databases
GENBANK
K02236
External Links
PubMed source DOI: 10.1128/mcb.4.7.1221-1230.1984 PMC Full Text View journal details
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