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PMID: 3453117 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Novel protein-tyrosine kinase gene (hck) preferentially expressed in cells of hematopoietic origin.

Molecular and cellular biology ·Vol. 7 ·No. 6 ·1987-06-00 ·Pages 2276-85

Ziegler SF, Marth JD, Lewis DB, Perlmutter RM

Abstract

Protein-tyrosine kinases are implicated in the control of cell growth by virtue of their frequent appearance as products of retroviral oncogenes and as components of growth factor receptors. Here we report the characterization of a novel human protein-tyrosine kinase gene (hck) that is primarily expressed in hematopoietic cells, particularly granulocytes. The hck gene encodes a 505-residue polypeptide that is closely related to pp56lck, a lymphocyte-specific protein-tyrosine kinase. The exon breakpoints of the hck gene, partially defined by using murine genomic clones, demonstrate that hck is a member of the src gene family and has been subjected to strong selection pressure during mammalian evolution. High-level expression of hck transcripts in granulocytes is especially provocative since these cells are terminally differentiated and typically survive in vivo for only a few hours. Thus the hck gene, like other members of the src gene family, appears to function primarily in cells with little growth potential.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Evolution Cell Line Cloning, Molecular DNA/metabolism Genes Humans Leukocytes/enzymology Lymphocytes/enzymology Mice Protein Biosynthesis Protein-Tyrosine Kinases/genetics
Chemicals
DNA Protein-Tyrosine Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ziegler S F
Marth J D
Lewis D B
Perlmutter R M
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56 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.6.2276-2285.1987
Published
1987-06-00
Pages
2276-85
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365352
Subset
IM
Databases
GENBANK
M16592
External Links
PubMed source DOI: 10.1128/mcb.7.6.2276-2285.1987 PMC Full Text View journal details
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