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PMID: 6441887 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Myristic acid is attached to the transforming protein of Rous sarcoma virus during or immediately after synthesis and is present in both soluble and membrane-bound forms of the protein.

Molecular and cellular biology ·Vol. 4 ·No. 12 ·1984-12-00 ·Pages 2697-704

Buss JE, Kamps MP, Sefton BM

Abstract

Myristic acid, a minor component of cellular fatty acids, has been shown previously to be covalently bound to most molecules of p60src, the transforming protein of Rous sarcoma virus. We have now determined at what time during the life cycle of p60src, and where within the cell, this lipid becomes attached to the protein. p60src was found to acquire myristic acid at only one time, during or immediately after its synthesis. p60src is known to be synthesized on free polysomes and appears at the cytoplasmic face of the plasma membrane after a lag of 10 min. The addition of myristic acid to p60src therefore precedes the binding of the protein to the plasma membrane. The lipid attached to p60src is a permanent, metabolically stable part of the protein; we found no evidence for turnover of the myristyl moiety. However, we did find myristate attached to various soluble forms of p60src and to a large number of cytosolic cellular proteins as well. This demonstrates that the attachment of myristic acid to a protein is not in itself sufficient to convert a soluble protein into a membrane-bound protein.

MeSH Terms
Animals Chick Embryo Cycloheximide/pharmacology Cytoplasm/analysis Electrophoresis, Polyacrylamide Gel Myristic Acid Myristic Acids/metabolism Oncogene Protein pp60(v-src) Solubility Viral Proteins/analysis,biosynthesis
Chemicals
Myristic Acids Viral Proteins Myristic Acid Cycloheximide Oncogene Protein pp60(v-src)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Buss J E
Kamps M P
Sefton B M
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42 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.12.2697-2704.1984
Published
1984-12-00
Pages
2697-704
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369279
Subset
IM
Grants
NCI NIH HHS · CA-14195 · United States
NCI NIH HHS · CA-17289 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.12.2697-2704.1984 PMC Full Text View journal details
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