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PMID: 3561390 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck.

Molecular and cellular biology ·Vol. 7 ·No. 1 ·1987-01-00 ·Pages 237-43

Yamanashi Y, Fukushige S, Semba K, Sukegawa J, Miyajima N, Matsubara K, Yamamoto T, Toyoshima K

Abstract

With v-yes DNA as the probe, a human cDNA library made from placental RNA was screened under relaxed conditions, and DNA clones derived from a novel genetic locus, termed lyn, were obtained. Nucleotide sequencing revealed that lyn could encode a novel tyrosine kinase that was very similar to mouse T-lymphocyte-specific tyrosine kinase p56lck and the v-yes protein as well as to the gene products of v-fgr and v-src. Northern hybridization analysis revealed that a 3.2-kilobase lyn mRNA was expressed in a variety of tissues of the human fetus. The pattern of lyn mRNA expression was different from those of related genes, such as yes and syn. Hybridization analysis of DNA from sorted chromosomes showed that the lyn gene is located on human chromosome 8 q13-qter.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Line Cloning, Molecular DNA/metabolism Female Genes Humans Placenta/enzymology Protein-Tyrosine Kinases/genetics Proto-Oncogene Proteins c-yes Retroviridae Proteins/genetics Retroviridae Proteins, Oncogenic
Chemicals
Retroviridae Proteins Retroviridae Proteins, Oncogenic DNA Protein-Tyrosine Kinases Proto-Oncogene Proteins c-yes YES1 protein, human
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Yamanashi Y
Fukushige S
Semba K
Sukegawa J
Miyajima N
Matsubara K
Yamamoto T
Toyoshima K
References (51)
51 references, click to expand
  1. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  2. Structure and biological activity of human homologs of the raf/mil oncogene.
    Mol Cell Biol. 1985 Jun;5(6):1400-7 PMID: 2993863
  3. A catalogue of splice junction sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
  4. Avian sarcoma virus Y73 genome sequence and structural similarity of its transforming gene product to that of Rous sarcoma virus.
    Nature. 1982 May 20;297(5863):205-8 PMID: 6281656
  5. Insulin stimulates tyrosine phosphorylation of the insulin receptor in a cell-free system.
    Nature. 1982 Aug 12;298(5875):667-9 PMID: 6178977
  6. Platelet-derived growth factor stimulates tyrosine-specific protein kinase activity in Swiss mouse 3T3 cell membranes.
    Proc Natl Acad Sci U S A. 1982 Jul;79(14):4303-7 PMID: 6181505
  7. Nucleotide sequence of Fujinami sarcoma virus: evolutionary relationship of its transforming gene with transforming genes of other sarcoma viruses.
    Cell. 1982 Oct;30(3):787-95 PMID: 6291784
  8. n-Tetradecanoyl is the NH2-terminal blocking group of the catalytic subunit of cyclic AMP-dependent protein kinase from bovine cardiac muscle.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6128-31 PMID: 6959104
  9. Identification of the NH2-terminal blocking group of calcineurin B as myristic acid.
    FEBS Lett. 1982 Dec 27;150(2):314-8 PMID: 7160476
  10. Structure and sequence of the cellular gene homologous to the RSV src gene and the mechanism for generating the transforming virus.
    Cell. 1983 Mar;32(3):881-90 PMID: 6299580
  11. Myristyl amino-terminal acylation of murine retrovirus proteins: an unusual post-translational proteins modification.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):339-43 PMID: 6340098
  12. Nucleotide sequence of Abelson murine leukemia virus genome: structural similarity of its transforming gene product to other onc gene products with tyrosine-specific kinase activity.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3623-7 PMID: 6304726
  13. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  14. Somatomedin-C stimulates the phosphorylation of the beta-subunit of its own receptor.
    J Biol Chem. 1983 Aug 25;258(16):9581-4 PMID: 6309774
  15. The erbB gene of avian erythroblastosis virus is a member of the src gene family.
    Cell. 1983 Nov;35(1):71-8 PMID: 6313229
  16. Gene product of v-fgr onc: hybrid protein containing a portion of actin and a tyrosine-specific protein kinase.
    Science. 1984 Jan 6;223(4631):63-6 PMID: 6318314
  17. The mouse c-abl locus: molecular cloning and characterization.
    Cell. 1984 Feb;36(2):349-56 PMID: 6319018
  18. A simple and very efficient method for generating cDNA libraries.
    Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
  19. Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences.
    Nature. 1984 Feb 9-15;307(5951):521-7 PMID: 6320011
  20. Nucleotide sequence of the feline retroviral oncogene v-fms shows unexpected homology with oncogenes encoding tyrosine-specific protein kinases.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):85-9 PMID: 6582485
  21. Point mutations close to the AUG initiator codon affect the efficiency of translation of rat preproinsulin in vivo.
    Nature. 1984 Mar 15-21;308(5956):241-6 PMID: 6700727
  22. Molecular cloning and characterization of avian sarcoma virus UR2 and comparison of its transforming sequence with those of other avian sarcoma viruses.
    J Virol. 1984 Jun;50(3):914-21 PMID: 6328022
  23. Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells.
    Nature. 1984 May 31-Jun 6;309(5967):418-25 PMID: 6328312
  24. Direct evidence that oncogenic tyrosine kinases and cyclic AMP-dependent protein kinase have homologous ATP-binding sites.
    Nature. 1984 Aug 16-22;310(5978):589-92 PMID: 6431300
  25. Evidence for two distinct c-src loci on human chromosomes 1 and 20.
    Nature. 1984 Nov 1-7;312(5989):70-1 PMID: 6092965
  26. Myristic acid, a rare fatty acid, is the lipid attached to the transforming protein of Rous sarcoma virus and its cellular homolog.
    J Virol. 1985 Jan;53(1):7-12 PMID: 2981363
  27. Amino terminal myristylation of the protein kinase p60src, a retroviral transforming protein.
    Science. 1985 Jan 25;227(4685):427-9 PMID: 3917576
  28. Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes.
    Nature. 1985 Feb 28-Mar 6;313(6005):756-61 PMID: 2983222
  29. Location of the c-yes gene on the human chromosome and its expression in various tissues.
    Science. 1985 Mar 1;227(4690):1038-40 PMID: 2983418
  30. Nucleotide sequence and topography of chicken c-fps. Genesis of a retroviral oncogene encoding a tyrosine-specific protein kinase.
    J Mol Biol. 1985 Jan 20;181(2):175-86 PMID: 3879969
  31. The product of the c-fms proto-oncogene: a glycoprotein with associated tyrosine kinase activity.
    Science. 1985 Apr 19;228(4697):320-2 PMID: 2580348
  32. Transcription termination and 3' processing: the end is in site!
    Cell. 1985 Jun;41(2):349-59 PMID: 2580642
  33. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  34. Human cellular src gene: nucleotide sequence and derived amino acid sequence of the region coding for the carboxy-terminal two-thirds of pp60c-src.
    Mol Cell Biol. 1985 May;5(5):1122-9 PMID: 2582238
  35. The c-fms proto-oncogene product is related to the receptor for the mononuclear phagocyte growth factor, CSF-1.
    Cell. 1985 Jul;41(3):665-76 PMID: 2408759
  36. Protein-tyrosine kinases.
    Annu Rev Biochem. 1985;54:897-930 PMID: 2992362
  37. A lymphocyte-specific protein-tyrosine kinase gene is rearranged and overexpressed in the murine T cell lymphoma LSTRA.
    Cell. 1985 Dec;43(2 Pt 1):393-404 PMID: 2416464
  38. Chromosomal assignment of human genes for gastrin, thyrotropin (TSH)-beta subunit and C-erbB-2 by chromosome sorting combined with velocity sedimentation and Southern hybridization.
    Biochem Biophys Res Commun. 1986 Jan 29;134(2):477-83 PMID: 3511905
  39. Expression of a new tyrosine protein kinase is stimulated by retrovirus promoter insertion.
    Nature. 1986 Feb 20-26;319(6055):682-5 PMID: 3081813
  40. Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP.
    Nucleic Acids Res. 1986 Feb 11;14(3):1319-24 PMID: 3951988
  41. Tyr527 is phosphorylated in pp60c-src: implications for regulation.
    Science. 1986 Mar 21;231(4744):1431-4 PMID: 2420005
  42. yes-related protooncogene, syn, belongs to the protein-tyrosine kinase family.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5459-63 PMID: 3526330
  43. Structure, expression, and chromosomal location of the human c-fgr gene.
    Mol Cell Biol. 1986 Feb;6(2):511-7 PMID: 3023853
  44. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
    Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
  45. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  46. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  47. Epidermal growth factor-receptor-protein kinase interactions. Co-purification of receptor and epidermal growth factor-enhanced phosphorylation activity.
    J Biol Chem. 1980 May 25;255(10):4834-42 PMID: 6246084
  48. A new human diploid cell strain, TIG-1, for the research on cellular aging.
    Exp Gerontol. 1980;15(2):121-33 PMID: 7389832
  49. Plasmid screening at high colony density.
    Gene. 1980 Jun;10(1):63-7 PMID: 6997135
  50. Characterization of cDNA clones for the human c-yes gene.
    Mol Cell Biol. 1987 Jan;7(1):41-7 PMID: 2436037
  51. Characterization of sites for tyrosine phosphorylation in the transforming protein of Rous sarcoma virus (pp60v-src) and its normal cellular homologue (pp60c-src).
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6013-7 PMID: 6273838
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.1.237-243.1987
Published
1987-01-00
Pages
237-43
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365062
Subset
IM
Databases
GENBANK
M16038
External Links
PubMed source DOI: 10.1128/mcb.7.1.237-243.1987 PMC Full Text View journal details
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