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PMID: 2582238 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Human cellular src gene: nucleotide sequence and derived amino acid sequence of the region coding for the carboxy-terminal two-thirds of pp60c-src.

Molecular and cellular biology ·Vol. 5 ·No. 5 ·1985-05-00 ·Pages 1122-9

Anderson SK, Gibbs CP, Tanaka A, Kung HJ, Fujita DJ

Abstract

The nucleotide sequence of the 3' two-thirds of a highly conserved, molecularly cloned human cellular src gene (c-src) has been determined. This region of the c-src gene encodes the tyrosine kinase domain of the cellular src protein (pp60c-src) and corresponds to exons 6 through 12 of the chicken c-src gene, as well as nucleotides 545 to 1542 of the Rous sarcoma virus src gene (v-src). The human c-src sequence is very strongly conserved with respect to both the chicken c-src and the Rous sarcoma virus v-src genes, with nearly 90% nucleotide homology observed in this region. Amino acid sequence conservation in this region is even greater; 98% of the amino acids are conserved between human and chicken c-src. Furthermore, the exon sizes and the locations of the exon-intron boundaries are identical in the human and chicken c-src genes. However, sequences within the introns have not been conserved, and the introns within the human c-src gene are significantly larger than the corresponding introns within the chicken c-src gene. The strong amino acid conservation between the carboxy-terminal two-thirds of pp60c-src of species as divergent as humans and chickens suggests that this portion of the pp60c-src protein specifies one or more functional domains that are of great importance to some aspect of normal cellular growth or differentiation.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Evolution Cloning, Molecular Genes Humans Oncogenes Phosphoproteins/genetics Protein Kinases/genetics Protein-Tyrosine Kinases Proto-Oncogene Proteins pp60(c-src)
Chemicals
Phosphoproteins Protein Kinases Protein-Tyrosine Kinases Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Anderson S K
Gibbs C P
Tanaka A
Kung H J
Fujita D J
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52 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.5.1122-1129.1985
Published
1985-05-00
Pages
1122-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366830
Subset
IM
Databases
GENBANK
K03212, K03213, K03214, K03215, K03216, K03217, K03218
External Links
PubMed source DOI: 10.1128/mcb.5.5.1122-1129.1985 PMC Full Text View journal details
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