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PMID: 6095204 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The nucleotide sequence of the B gene of bacteriophage Mu.

Nucleic acids research ·Vol. 12 ·No. 22 ·1984-11-26 ·Pages 8627-38

Miller JL, Anderson SK, Fujita DJ, Chaconas G, Baldwin DL, Harshey RM

Abstract

Bacteriophage Mu is a highly efficient transposon which requires the products of the Mu A and B genes in order to transpose at a normal frequency. We have determined the nucleotide sequence of the B gene as well as that of the A-B intergenic region upstream of B. The protein product of the gene contains 312 amino acids and has a predicted molecular weight of 35,061. As expected, there do not appear to be any potential promoter sequences in the intergenic region prior to the gene, but it is preceded by a strong Shine-Dalgarno sequence. The intergenic region does not contain any obvious transcription termination sequences. The frequency of optimal codon usage is similar to that for other transposon and phage genes, and the amino acid composition is comparable to that of an "average" E. coli protein. A region near the amino terminus of the protein resembles the highly conserved bihelical fold which is involved in DNA contact and sequence specific recognition in a number of DNA binding proteins.

MeSH Terms

Amino Acid Sequence Base Sequence Cloning, Molecular Coliphages/genetics DNA Restriction Enzymes Escherichia coli/genetics Genes, Bacterial Genes, Viral Plasmids Viral Proteins/genetics

Chemicals

Viral Proteins DNA Restriction Enzymes

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Miller J L

Anderson S K

Fujita D J

Chaconas G

Baldwin D L

Harshey R M

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26 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.22.8627

Published

1984-11-26

Pages

8627-38

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320403

Subset

Grants

NIGMS NIH HHS · GM33247-01 · United States

Databases

GENBANK

K03390

External Links

PubMed source DOI: 10.1093/nar/12.22.8627 PMC Full Text View journal details

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The nucleotide sequence of the B gene of bacteriophage Mu.

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