Bacteriophage Mu DNA replication is stimulated by non-essential early functions.

Molecular & general genetics : MGG ·Vol. 186 ·No. 1 ·1982-00-00 ·Pages 135-9

Goosen T, Giphart-Gassler M, Van de Putte P

Abstract

The replication of a spontaneous Kil- mutant of bacteriophage Mu has been investigated. The Kil- mutation (Mucts62-13/4), which was introduced into a defective prophage, is pleiotrophic, leading to the loss of also the Gam, Cim and Sot functions. The mutation is caused by an insertion with the characteristics of IS1, located just outside the B gene. Mucts62-1 3/4 phages form extremely small plaques on wildtype indicator strains. The replication of the insertion mutant as compared to Mucts62 is strongly reduced. Normal replication could be restored by relieving the polarity of the insertion or by complementation with defective prophages which express all early functions. Apparently, early genes other than A and B are involved in normal Mu DNA replication.

MeSH Terms

Coliphages/genetics DNA Replication DNA, Viral/isolation & purification Escherichia coli/genetics Kinetics Lysogeny Nucleic Acid Hybridization Species Specificity Temperature Virus Replication

Chemicals

DNA, Viral

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Goosen T

Giphart-Gassler M

Van de Putte P

References (17)

17 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00422925

Published

1982-00-00

Pages

135-9

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00422925

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Bacteriophage Mu DNA replication is stimulated by non-essential early functions.

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