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PMID: 3011421 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Activation of the pp60c-src kinase during differentiation of monomyelocytic cells in vitro.

The EMBO journal ·Vol. 5 ·No. 4 ·1986-04-00 ·Pages 701-5

Barnekow A, Gessler M

Abstract

The proto-oncogene c-src, the cellular homolog of the Rous sarcoma virus (RSV) transforming gene v-src, is expressed in a tissue-specific and age-dependent manner. Its physiological function, although still unknown, appears to be more closely related to differentiation processes than to proliferation processes. To obtain more information about the physiological role of the c-src gene in cells, we have studied differentiation-dependent alterations using the human HL-60 leukaemia cell line as a model system. Induction of monocytic and granulocytic differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) and dimethylsulfoxide (DMSO) is associated with an activation of the pp60c-src tyrosine kinase, but not with increased c-src gene expression. Control experiments exclude an interaction of TPA and DMSO themselves with the pp60c-src kinase.

MeSH Terms
Avian Sarcoma Viruses/genetics Cell Differentiation Cell Line Enzyme Activation Enzyme Induction Genes Genes, Viral Humans Kinetics Leukemia, Myeloid, Acute Oncogene Protein pp60(v-src) Phosphorylation Protein Kinases/biosynthesis Proto-Oncogene Mas Proto-Oncogenes Retroviridae Proteins/biosynthesis,genetics Tetradecanoylphorbol Acetate/pharmacology
Chemicals
MAS1 protein, human Proto-Oncogene Mas Retroviridae Proteins Protein Kinases Oncogene Protein pp60(v-src) Tetradecanoylphorbol Acetate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Barnekow A
Gessler M
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42 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-04-00
Pages
701-5
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1166847
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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