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PMID: 2425362 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Differentiation of myeloid cells is accompanied by increased levels of pp60c-src protein and kinase activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 14 ·1986-07-00 ·Pages 5131-5

Gee CE, Griffin J, Sastre L, Miller LJ, Springer TA, Piwnica-Worms H, Roberts TM

Abstract

We have detected a significant increase in the levels of pp60c-src kinase activity associated with the differentiation of myeloid cell lines HL-60 and U-937. The induction of pp60c-src kinase activity becomes apparent approximately 14 hr after the addition of phorbol 12-myristate 13-acetate and increases 20-fold by 72 hr. The enhanced kinase activity can be accounted for by elevated levels of c-src protein in the differentiated cells. When nonleukemic bone marrow cells were examined, myeloid progenitor cells exhibited a low level of pp60c-src kinase activity. As these cells are allowed to differentiate in culture, the resulting adherent monocytes are as high in pp60c-src kinase activity as HL-60 cells induced to differentiate into monocytes. A strong correlation is found between the levels of pp60c-src kinase activity and the degree of monocytic differentiation of the cells from patients with acute myeloid leukemia. Our findings suggest that the activation of pp60c-src kinase activity is a normal physiological event associated with myeloid differentiation.

MeSH Terms
Cell Differentiation/drug effects Cell Line Humans Leukemia, Monocytic, Acute/pathology Leukemia, Myeloid, Acute/pathology Male Monocytes/pathology Neoplasm Proteins/metabolism Phosphorylation Protein-Tyrosine Kinases/metabolism Proto-Oncogene Proteins/metabolism Proto-Oncogene Proteins pp60(c-src) Tetradecanoylphorbol Acetate/pharmacology
Chemicals
Neoplasm Proteins Proto-Oncogene Proteins Protein-Tyrosine Kinases Proto-Oncogene Proteins pp60(c-src) Tetradecanoylphorbol Acetate
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Gee C E
Griffin J
Sastre L
Miller L J
Springer T A
Piwnica-Worms H
Roberts T M
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.14.5131
Published
1986-07-00
Pages
5131-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323904
Subset
IM
Grants
NIAID NIH HHS · AI106913 · United States
NIAID NIH HHS · AI20316 · United States
NCI NIH HHS · CA-36167-02 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.14.5131 PMC Full Text View journal details
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