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PMID: 2424008 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Phosphatidylinositol metabolism and polyoma-mediated transformation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 11 ·1986-06-00 ·Pages 3624-8

Kaplan DR, Whitman M, Schaffhausen B, Raptis L, Garcea RL, Pallas D, Roberts TM, Cantley L

Abstract

The effect of polyoma middle-sized tumor antigen (MTAg) on phosphatidylinositol metabolism has been characterized in vivo and in vitro using polyoma-transformed and polyoma-infected cells. Cells infected with transformation-competent polyoma virus exhibit increased levels of inositol phospholipids and the second messenger inositol trisphosphate. MTAg or pp60c-src immunoprecipitates from MTAg-transformed cells contain an activity that phosphorylates phosphatidylinositol and phosphatidylinositol 4-phosphate. This activity is induced in parallel with MTAg when the MTAg synthesis is regulated by hormonal or heavy metal inducers. Immunoprecipitates from one class of polyoma mutants defective in transformation have a reduced level of associated phosphatidylinositol kinase activity in vitro yet are capable of tyrosine phosphorylation on exogenous protein substrates at rates comparable to wild-type virus. Thus, for these mutants, phosphatidylinositol kinase activity is more tightly correlated with transformation than is protein kinase activity. These results suggest that alterations in phosphatidylinositol metabolism by MTAg play a role in transformation by polyoma virus.

MeSH Terms
Animals Antigens, Viral, Tumor/genetics Cell Transformation, Viral Mice Mutation Phosphatidylinositols/metabolism Phosphorylation Polyomavirus/immunology,physiology Protein-Tyrosine Kinases/metabolism Proto-Oncogene Proteins/metabolism Proto-Oncogene Proteins pp60(c-src) Rats
Chemicals
Antigens, Viral, Tumor Phosphatidylinositols Proto-Oncogene Proteins Protein-Tyrosine Kinases Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Kaplan D R
Whitman M
Schaffhausen B
Raptis L
Garcea R L
Pallas D
Roberts T M
Cantley L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.11.3624
Published
1986-06-00
Pages
3624-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323575
Subset
IM
Grants
NCI NIH HHS · CA34722 · United States
NIGMS NIH HHS · GM36133 · United States
NCI NIH HHS · CA3002 · United States
NCI NIH HHS · R01 CA037667 · United States
NCI NIH HHS · R01 CA034722 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.11.3624 PMC Full Text View journal details
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