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PMID: 3927285 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Tyrosine phosphorylation within the amino-terminal domain of pp60c-src molecules associated with polyoma virus middle-sized tumor antigen.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 14 ·1985-07-00 ·Pages 4568-72

Yonemoto W, Jarvis-Morar M, Brugge JS, Bolen JB, Israel MA

Abstract

We have examined the in vitro phosphorylation of cellular src protein (pp60c-src) molecules associated with the polyoma virus middle-sized tumor antigen in polyoma virus-transformed cells. These pp60c-src molecules possessed an enhanced tyrosyl kinase activity, migrated aberrantly on NaDodSO4/polyacrylamide gels, and contained a novel site of tyrosine phosphorylation within the amino-terminal region of the molecule. The pp60c-src molecules not associated with the middle-sized tumor antigen were phosphorylated exclusively on a tyrosine residue within the carboxyl-terminal domain of pp60c-src. A similar modified form of the middle-sized tumor antigen-associated pp60c-src protein was detected in lysates from polyoma virus-transformed cells labeled in vivo with [32P]orthophosphate in the presence of sodium orthovanadate, an inhibitor of phosphotyrosyl phosphatases.

MeSH Terms
Animals Antibodies, Monoclonal Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/metabolism Cell Line Cells, Cultured Oncogene Protein pp60(v-src) Peptide Fragments/analysis Phosphorylation Protein Kinases/metabolism Rats Rats, Inbred F344 Transfection Tyrosine Viral Proteins/metabolism
Chemicals
Antibodies, Monoclonal Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Peptide Fragments Viral Proteins Tyrosine Protein Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yonemoto W
Jarvis-Morar M
Brugge J S
Bolen J B
Israel M A
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.14.4568
Published
1985-07-00
Pages
4568-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390426
Subset
IM
Grants
NCI NIH HHS · CA27951-05 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.14.4568 PMC Full Text View journal details
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