Inhibition of human alkaline phosphatases by vanadate.

The Biochemical journal ·Vol. 181 ·No. 1 ·1979-07-01 ·Pages 247-50

Seargeant LE, Stinson RA

Abstract

Orthovanadate was shown to be a potent competitive inhibitor (Ki less than 1 microM) of purified alkaline phosphatase from human liver, intestine of kidney. Inhibition was reversed and full enzymic activity restored in the presence of 1mM-adrenaline. Phosphate and vanadate competed for the same binding site on the enzyme.

MeSH Terms

Alkaline Phosphatase/antagonists & inhibitors Humans Intestine, Small/enzymology Kidney/enzymology Kinetics Liver/enzymology Phosphates/pharmacology Vanadium/pharmacology

Chemicals

Phosphates Vanadium Alkaline Phosphatase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Seargeant L E

Stinson R A

References (18)

18 references, click to expand

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Vanadate is a potent (Na,K)-ATPase inhibitor found in ATP derived from muscle.
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A convenient method for the ATPase assay.
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1810247

Published

1979-07-01

Pages

247-50

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1161148

Subset

External Links

PubMed source DOI: 10.1042/bj1810247 PMC Full Text View journal details

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Inhibition of human alkaline phosphatases by vanadate.

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