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PMID: 7784171 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of the Saccharomyces cerevisiae DNA repair gene RAD16.

Nucleic acids research ·Vol. 23 ·No. 10 ·1995-05-25 ·Pages 1679-85

Bang DD, Timmermans V, Verhage R, Zeeman AM, van de Putte P, Brouwer J

Abstract

The RAD16 gene product has been shown to be essential for the repair of the silenced mating type loci [Bang et al. (1992) Nucleic Acids Res. 20, 3925-3931]. More recently we demonstrated that the RAD16 and RAD7 proteins are also required for repair of non-transcribed strands of active genes in Saccharomyces cerevisiae [Waters et al. (1993) Mol. Gen. Genet. 239, 28-32]. We have studied the regulation of the RAD16 gene and found that the RAD16 transcript levels increased up to 7-fold upon UV irradiation. Heat shock at 42 degrees C also results in elevated levels of RAD16 mRNA. In sporulating MAT alpha/MATa diploid cells RAD16 mRNA is also induced. The basal level of the RAD16 transcript is constant during the mitotic cell cycle. G1-arrested cells show normal induction of RAD16 mRNA upon UV irradiation demonstrating that the induction is not a secondary consequence of G2 cell cycle arrest following UV irradiation. However, in cells arrested in G1 the induction of RAD16 mRNA after UV irradiation is not followed by a rapid decline as occurs in normal growing cells suggesting that the down regulation of RAD16 transcription is dependent on progression into the cell cycle.

Related Genes
RAD16
MeSH Terms
Adenosine Triphosphatases Cell Cycle DNA Repair/genetics,radiation effects DNA, Fungal/biosynthesis,isolation & purification DNA-Binding Proteins Fungal Proteins/biosynthesis,genetics,metabolism Gene Expression Regulation, Fungal/radiation effects Genes, Fungal Genes, Mating Type, Fungal Kinetics Meiosis Mitosis RNA, Messenger/analysis,biosynthesis Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins Spores, Fungal/physiology Time Factors Ultraviolet Rays
Chemicals
DNA, Fungal DNA-Binding Proteins Fungal Proteins RAD7 protein, S cerevisiae RNA, Messenger Saccharomyces cerevisiae Proteins Adenosine Triphosphatases RAD16 protein, S cerevisiae
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bang D D
Department of Biochemistry, Leiden Institute for Chemistry, Gorlaeus Laboratories, Leiden University, The Netherlands.
Timmermans V
Verhage R
Zeeman A M
van de Putte P
Brouwer J
References (42)
42 references, click to expand
  1. Expression of the yeast UB14 gene increases in response to DNA-damaging agents and in meiosis.
    Mol Cell Biol. 1988 Mar;8(3):1132-6 PMID: 2835662
  2. Cloning the RAD51 homologue of Schizosaccharomyces pombe.
    Nucleic Acids Res. 1993 Sep 25;21(19):4586-91 PMID: 8233794
  3. Identification of the gene for the yeast ribonucleotide reductase small subunit and its inducibility by methyl methanesulfonate.
    Mol Cell Biol. 1987 Oct;7(10):3673-7 PMID: 3316984
  4. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.
    Bacteriol Rev. 1976 Dec;40(4):869-907 PMID: 795416
  5. Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation.
    Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2687-91 PMID: 1901413
  6. Molecular cloning of RAD16, a gene involved in differential repair in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1992 Aug 11;20(15):3925-31 PMID: 1508678
  7. The SOS regulatory system of Escherichia coli.
    Cell. 1982 May;29(1):11-22 PMID: 7049397
  8. Identification and isolation of the gene encoding the small subunit of ribonucleotide reductase from Saccharomyces cerevisiae: DNA damage-inducible gene required for mitotic viability.
    Mol Cell Biol. 1987 Aug;7(8):2783-93 PMID: 3313004
  9. Failure to induce a DNA repair gene, RAD54, in Saccharomyces cerevisiae does not affect DNA repair or recombination phenotypes.
    Mol Cell Biol. 1989 Aug;9(8):3314-22 PMID: 2552291
  10. RAD9-dependent G1 arrest defines a second checkpoint for damaged DNA in the cell cycle of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):7985-9 PMID: 8367452
  11. Cloning and sequence analysis of the Saccharomyces cerevisiae RAD9 gene and further evidence that its product is required for cell cycle arrest induced by DNA damage.
    Mol Cell Biol. 1989 May;9(5):1882-96 PMID: 2664461
  12. ERCC6, a member of a subfamily of putative helicases, is involved in Cockayne's syndrome and preferential repair of active genes.
    Cell. 1992 Dec 11;71(6):939-53 PMID: 1339317
  13. Specific Saccharomyces cerevisiae genes are expressed in response to DNA-damaging agents.
    Mol Cell Biol. 1985 Jan;5(1):75-84 PMID: 3920512
  14. Expression of the Saccharomyces cerevisiae DNA repair gene RAD6 that encodes a ubiquitin conjugating enzyme, increases in response to DNA damage and in meiosis but remains constant during the mitotic cell cycle.
    Nucleic Acids Res. 1990 Feb 25;18(4):771-8 PMID: 2179869
  15. The yeast DNA polymerase I transcript is regulated in both the mitotic cell cycle and in meiosis and is also induced after DNA damage.
    Nucleic Acids Res. 1987 Jul 10;15(13):5017-30 PMID: 3299263
  16. The Saccharomyces cerevisiae DNA repair gene RAD2 is regulated in meiosis but not during the mitotic cell cycle.
    Mol Cell Biol. 1990 Jun;10(6):3256-7 PMID: 2188112
  17. The RAD9 gene controls the cell cycle response to DNA damage in Saccharomyces cerevisiae.
    Science. 1988 Jul 15;241(4863):317-22 PMID: 3291120
  18. The RAD7 and RAD16 genes, which are essential for pyrimidine dimer removal from the silent mating type loci, are also required for repair of the nontranscribed strand of an active gene in Saccharomyces cerevisiae.
    Mol Cell Biol. 1994 Sep;14(9):6135-42 PMID: 8065346
  19. Regulation of RAD54- and RAD52-lacZ gene fusions in Saccharomyces cerevisiae in response to DNA damage.
    Mol Cell Biol. 1987 Mar;7(3):1078-84 PMID: 3550429
  20. A damage-responsive DNA binding protein regulates transcription of the yeast DNA repair gene PHR1.
    Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11251-5 PMID: 1763039
  21. Regulation of CDC9, the Saccharomyces cerevisiae gene that encodes DNA ligase.
    Mol Cell Biol. 1985 Jan;5(1):226-35 PMID: 3885010
  22. Differential repair of UV damage in rad mutants of Saccharomyces cerevisiae: a possible function of G2 arrest upon UV irradiation.
    Mol Cell Biol. 1990 Sep;10(9):4678-84 PMID: 2201899
  23. Transcript levels of the Saccharomyes cerevisiae DNA repair gene RAD23 increase in response to UV light and in meiosis but remain constant in the mitotic cell cycle.
    Nucleic Acids Res. 1990 Aug 25;18(16):4737-42 PMID: 2204027
  24. Inducible DNA repair systems.
    Annu Rev Biochem. 1985;54:425-57 PMID: 3896123
  25. Chromosome tangling and breakage at anaphase result from mutations in lodestar, a Drosophila gene encoding a putative nucleoside triphosphate-binding protein.
    Genes Dev. 1991 Oct;5(10):1786-99 PMID: 1916263
  26. Control of vacuole permeability and protein degradation by the cell cycle arrest signal in Saccharomyces cerevisiae.
    J Bacteriol. 1978 Oct;136(1):234-46 PMID: 361691
  27. The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses.
    Cell. 1987 Mar 27;48(6):1035-46 PMID: 3030556
  28. X-ray-induced division delay of individual yeast cells.
    Radiat Res. 1956 May;4(5):394-412 PMID: 13323261
  29. Regulated expression of the Saccharomyces cerevisiae DNA repair gene RAD7 in response to DNA damage and during sporulation.
    Nucleic Acids Res. 1990 Jun 11;18(11):3281-5 PMID: 2192359
  30. Two DNA repair and recombination genes in Saccharomyces cerevisiae, RAD52 and RAD54, are induced during meiosis.
    Mol Cell Biol. 1989 Jul;9(7):3101-4 PMID: 2506437
  31. A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae.
    Nucleic Acids Res. 1990 May 25;18(10):3091-2 PMID: 2190191
  32. Deoxyribonucleic acid repair in the yeast Saccharomyces cerevisiae.
    Microbiol Rev. 1988 Mar;52(1):70-102 PMID: 3280967
  33. Nucleotide excision repair in Escherichia coli.
    Microbiol Rev. 1990 Mar;54(1):18-51 PMID: 2181258
  34. DNA damage and heat shock dually regulate genes in Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Jan;6(1):90-6 PMID: 3023840
  35. Differential repair of UV damage in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1989 Jun 26;17(12):4433-9 PMID: 2664708
  36. Inducible removal of UV-induced pyrimidine dimers from transcriptionally active and inactive genes of Saccharomyces cerevisiae.
    Mol Gen Genet. 1993 May;239(1-2):28-32 PMID: 8510656
  37. Specific transcripts are elevated in Saccharomyces cerevisiae in response to DNA damage.
    Mol Cell Biol. 1984 Nov;4(11):2356-63 PMID: 6440006
  38. Sequence of RAD54, a Saccharomyces cerevisiae gene involved in recombination and repair.
    Gene. 1991 Jul 31;104(1):103-6 PMID: 1916269
  39. Cloning and characterisation of the Schizosaccharomyces pombe rad8 gene, a member of the SNF2 helicase family.
    Nucleic Acids Res. 1993 Dec 25;21(25):5964-71 PMID: 8290359
  40. Transcript levels of the Saccharomyces cerevisiae DNA repair gene RAD18 increase in UV irradiated cells and during meiosis but not during the mitotic cell cycle.
    Nucleic Acids Res. 1991 Feb 25;19(4):893-8 PMID: 2017370
  41. Saccharomyces cerevisiae RAD5-encoded DNA repair protein contains DNA helicase and zinc-binding sequence motifs and affects the stability of simple repetitive sequences in the genome.
    Mol Cell Biol. 1992 Sep;12(9):3807-18 PMID: 1324406
  42. Nucleotide sequence, transcript mapping, and regulation of the RAD2 gene of Saccharomyces cerevisiae.
    J Bacteriol. 1986 Jun;166(3):914-23 PMID: 3011752
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/23.10.1679
Published
1995-05-25
Pages
1679-85
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306921
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/23.10.1679 PMC Full Text View journal details
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