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PMID: 2201899 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential repair of UV damage in rad mutants of Saccharomyces cerevisiae: a possible function of G2 arrest upon UV irradiation.

Molecular and cellular biology ·Vol. 10 ·No. 9 ·1990-09-00 ·Pages 4678-84

Terleth C, Schenk P, Poot R, Brouwer J, van de Putte P

Abstract

After UV irradiation, the transcriptionally active MAT alpha locus in Saccharomyces cerevisiae is preferentially repaired compared with the inactive HML alpha locus. The effect of rad mutations from three different epistasis groups on differential repair was investigated. Three mutants, rad9, rad16, and rad24, were impaired in the removal of UV dimers from the inactive HML alpha locus, whereas they had generally normal repair of the active MAT alpha locus. Since RAD9 is necessary for G2 arrest after UV irradiation, we propose that the G2 stage plays a role in making the dimers accessible for repair, at least in the repressed HML alpha locus.

MeSH Terms
Blotting, Southern DNA Damage DNA Repair DNA, Fungal/genetics,isolation & purification,radiation effects Genes, Fungal Genotype Interphase/radiation effects Kinetics Mutation Saccharomyces cerevisiae/cytology,genetics,radiation effects Time Factors Ultraviolet Rays
Chemicals
DNA, Fungal
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Terleth C
Department of Biochemistry, Gorlaeus Laboratories, Leiden University, The Netherlands.
Schenk P
Poot R
Brouwer J
van de Putte P
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.9.4678-4684.1990
Published
1990-09-00
Pages
4678-84
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361057
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/mcb.10.9.4678-4684.1990 PMC Full Text View journal details
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