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PMID: 2664708 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential repair of UV damage in Saccharomyces cerevisiae.

Nucleic acids research ·Vol. 17 ·No. 12 ·1989-06-26 ·Pages 4433-9

Terleth C, van Sluis CA, van de Putte P

Abstract

Preferential repair of UV-induced damage is a phenomenon by which mammalian cells might enhance their survival. This paper presents the first evidence that preferential repair occurs in the lower eukaryote Saccharomyces cerevisiae. Moreover an unique approach is reported to compare identical sequences present on the same chromosome and only differing in expression. We determined the removal of pyrimidine dimers from two identical alpha-mating type loci and we were able to show that the active MAT alpha locus is repaired preferentially to the inactive HML alpha locus. In a sir-3 mutant, in which both loci are active this preference is not observed.

MeSH Terms
DNA Damage DNA Repair DNA, Fungal/isolation & purification,radiation effects Deoxyribonucleases, Type II Site-Specific/radiation effects Dose-Response Relationship, Radiation Genes, Fungal/radiation effects Mutation Pyrimidine Dimers/radiation effects Saccharomyces cerevisiae/genetics,radiation effects Transcription, Genetic Ultraviolet Rays
Chemicals
DNA, Fungal Pyrimidine Dimers endodeoxyribonuclease HaeII Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Terleth C
Leiden University, Department of Molecular Genetics, The Netherlands.
van Sluis C A
van de Putte P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.12.4433
Published
1989-06-26
Pages
4433-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318003
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.12.4433 PMC Full Text View journal details
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