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PMID: 3920512 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Specific Saccharomyces cerevisiae genes are expressed in response to DNA-damaging agents.

Molecular and cellular biology ·Vol. 5 ·No. 1 ·1985-01-00 ·Pages 75-84

Ruby SW, Szostak JW

Abstract

When exposed to DNA-damaging agents, the yeast Saccharomyces cerevisiae induces the expression of at least six specific genes. We have previously identified one damage inducible (DIN) gene as a gene fusion (din-lacZ fusion) whose expression increases in response to DNA-damaging treatments. We describe here the identification of five additional DIN genes as din-lacZ fusions and the responses of all six DIN genes to DNA-damaging agents. Northern blot analyses of the transcripts of two of the DIN genes show that their levels increase after exposure to DNA-damaging agents. Five of the din-lacZ fusions are induced in S. cerevisiae cells exposed to UV light, gamma rays, methotrexate, or alkylating agents. One of the din-lacZ fusions is induced by either UV or methotrexate but not by the other agents. This finding suggests that there are sets of DIN genes that are regulated differently.

MeSH Terms
Alkylating Agents DNA Repair DNA, Fungal/genetics DNA, Recombinant Gene Expression Regulation/drug effects Genes, Fungal Kinetics Methyl Methanesulfonate/pharmacology Saccharomyces cerevisiae/genetics Transcription, Genetic/drug effects Ultraviolet Rays beta-Galactosidase/genetics
Chemicals
Alkylating Agents DNA, Fungal DNA, Recombinant Methyl Methanesulfonate beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ruby S W
Szostak J W
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44 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.1.75-84.1985
Published
1985-01-00
Pages
75-84
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366680
Subset
IM
Grants
NIGMS NIH HHS · 5 T32 GM07196-07 · United States
NCI NIH HHS · T32 CA 09361-03 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.1.75-84.1985 PMC Full Text View journal details
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