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PMID: 2017370 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transcript levels of the Saccharomyces cerevisiae DNA repair gene RAD18 increase in UV irradiated cells and during meiosis but not during the mitotic cell cycle.

Nucleic acids research ·Vol. 19 ·No. 4 ·1991-02-25 ·Pages 893-8

Jones JS, Prakash L

Abstract

We have examined the transcript levels of the Saccharomyces cerevisiae DNA repair gene RAD18 in UV irradiated cells, in the mitotic cell cycle, and during meiosis. Levels of RAD18 mRNA increased upon UV irradiation, but remained constant during the mitotic cell cycle. During meiosis, RAD18 mRNA levels rose about 4 fold at a stage coincident with the period when high levels of genetic recombination occur. RAD18 resembles the other DNA repair genes, RAD2, RAD6, RAD7, RAD23, and RAD54, all of which also exhibit increased transcription in response to DNA damage and during meiosis. Comparisons of sequences in 5' flanking regions of RAD genes suggest that different upstream sequences are involved in regulating the expression of DNA repair genes belonging to different epistasis groups.

Related Genes
RAD18
MeSH Terms
Base Sequence Blotting, Northern DNA Repair Electrophoresis, Polyacrylamide Gel Genes, Fungal Meiosis/radiation effects Mitosis/radiation effects Molecular Sequence Data Nucleic Acid Hybridization RNA, Messenger/analysis Saccharomyces cerevisiae/genetics Transcription, Genetic/radiation effects Ultraviolet Rays
Chemicals
RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jones J S
Department of Biophysics, University of Rochester School of Medicine, NY 14642.
Prakash L
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.4.893
Published
1991-02-25
Pages
893-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333727
Subset
IM
Grants
NIGMS NIH HHS · GM19261 · United States
External Links
PubMed source DOI: 10.1093/nar/19.4.893 PMC Full Text View journal details
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