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PMID: 7038396 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Characterization of postreplication repair in Saccharomyces cerevisiae and effects of rad6, rad18, rev3 and rad52 mutations.

Molecular & general genetics : MGG ·Vol. 184 ·No. 3 ·1981-00-00 ·Pages 471-8

Prakash L

Abstract

Postreplication repair of nuclear DNA was examined in an excision defective haploid strain of yeast lacking mitochondrial DNA (rad1 rho 0). The size of the DNA synthesized in cells exposed to various fluences of ultraviolet light (UV) corresponds approximately to the average interdimer distance in the parental DNA. Upon further incubation of cells following exposure to 2.5 J/m2, the DNA increases in size; by 4 h, it corresponds to DNA from uniformly labeled cells. The alkaline sucrose sedimentation pattern of DNA pulse labeled at various times after UV irradiation, for up to 4 h, does not change substantially, indicating that dimers continue to block DNA replication. A significant amount of postreplication repair requires de novo protein synthesis, as determined by its inhibition by cycloheximide. The rad6 mutant does not carry out postreplication repair, the rad18 and rad52 mutants show great inhibition while the rev3 mutation does not affect postreplication repair. Both recombinational and nonrecombinational repair mechanisms may function in postreplication repair and most of postreplication repair is error free.

MeSH Terms
Cycloheximide/pharmacology DNA Repair DNA Replication/drug effects,radiation effects DNA, Fungal/radiation effects Kinetics Molecular Weight Mutation Saccharomyces cerevisiae/drug effects,genetics,radiation effects Spheroplasts/radiation effects Ultraviolet Rays
Chemicals
DNA, Fungal Cycloheximide
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Prakash L
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50 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00352525
Published
1981-00-00
Pages
471-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM19261 · United States
External Links
PubMed source DOI: 10.1007/BF00352525
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