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PMID: 7621834 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Unwinding of chromatin by the SV40 large T antigen DNA helicase.

The EMBO journal ·Vol. 14 ·No. 13 ·1995-07-03 ·Pages 3215-25

Ramsperger U, Stahl H

Abstract

We have analysed the unwinding of nucleosomally organized DNA by simian virus 40 large tumour (T) antigen. Isolated T antigen can bind to existing nucleosome cores containing the viral replication origin sequence, which results in displacement of the histone octamer and unwinding of the DNA. However, specific binding to nucleosome cores is salt sensitive and nearly completely blocked under ionic conditions that otherwise support DNA replication. Once started, the progressing T antigen helicase, like an elongating RNA polymerase, is not further repressed by histone octamers, irrespective of the presence or absence of linker histone H1. Disruption of the nucleosomal structure in the process of unwinding may be assisted by the demonstrated interaction of the hexameric T antigen complex with histone proteins H1 and H3. Finally, our studies reveal the inability of topoisomerase I and/or II to continually relieve the superhelical tension of covalently closed circular minichromosomes as generated during their unwinding by T antigen. This may indicate that chromatin relaxation during the process of DNA replication can only be efficiently performed by a topoisomerase that is (trans)activated by other factors.

MeSH Terms
Adenosine Triphosphate/chemistry Animals Antigens, Polyomavirus Transforming/chemistry Chromatin/chemistry Chromosomes/chemistry DNA/biosynthesis DNA Helicases/chemistry DNA-Binding Proteins/chemistry Female Histones/chemistry Nucleic Acid Conformation Nucleosomes/chemistry Oocytes Replication Origin/genetics Sodium Chloride/chemistry Spodoptera Xenopus laevis
Chemicals
Antigens, Polyomavirus Transforming Chromatin DNA-Binding Proteins Histones Nucleosomes Sodium Chloride Adenosine Triphosphate DNA DNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ramsperger U
Fakultät für Biologie, Universität Konstanz, Germany.
Stahl H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-07-03
Pages
3215-25
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394383
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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