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PMID: 2153220 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.

Journal of virology ·Vol. 64 ·No. 2 ·1990-02-00 ·Pages 509-18

Parsons R, Anderson ME, Tegtmeyer P

Abstract

The simian virus 40 (SV40) core origin of replication consists of three functional domains. The sequence 5'-CACTACTTCTGGAATAG-3' with an imperfect inverted repeat (underlined), a palindrome with four 5'-GAGGC-3' pentanucleotide repeats, and a 17-base-pair A + T-rich segment. We have been able to assign primary functions to each domain. Remarkably, SV40 large T antigen melted the inverted repeat domain in the complete absence of other origin sequences. Presumably, this protein-DNA interaction initiates a replication bubble that leads to daughter strand DNA synthesis. The pentanucleotide domain alone docked and arranged T antigen at the origin. The A + T-rich domain had no independent function, but, in the presence of the other two domains, allowed bound T antigen to extend the replication bubble. Thus, three domains of the origin coordinate the binding, melting, and DNA helicase activities of T antigen in an ordered sequence of events to initiate DNA replication.

MeSH Terms
Antigens, Polyomavirus Transforming/genetics,isolation & purification Base Sequence DNA Helicases/genetics,isolation & purification,metabolism DNA Replication DNA, Viral/genetics Deoxyribonuclease I Genes, Viral Kinetics Molecular Sequence Data Mutation Nucleotide Mapping Recombinant Proteins/isolation & purification,metabolism Repetitive Sequences, Nucleic Acid Simian virus 40/genetics,immunology
Chemicals
Antigens, Polyomavirus Transforming DNA, Viral Recombinant Proteins Deoxyribonuclease I DNA Helicases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Parsons R
Department of Microbiology, State University of New York, Stony Brook 11794-8621.
Anderson M E
Tegtmeyer P
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.2.509-518.1990
Published
1990-02-00
Pages
509-18
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249138
Subset
IM
Grants
NCI NIH HHS · CA-09176 · United States
NCI NIH HHS · CA-18808 · United States
NCI NIH HHS · CA-28146 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.2.509-518.1990 PMC Full Text View journal details
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