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PMID: 2829177 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

ATP stimulates the binding of simian virus 40 (SV40) large tumor antigen to the SV40 origin of replication.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 1 ·1988-01-00 ·Pages 64-8

Borowiec JA, Hurwitz J

Abstract

Simian virus 40 (SV40) large tumor antigen (T antigen) binds to two contiguous sites at the SV40 origin of replication. Of these two sites, I and II, only site II is critical for replication. We have studied the interaction between T antigen and these sites by two methods--nitrocellulose filter binding and DNase I protection. We show that T antigen binds with high occupancy to site I at 0 degrees C, 25 degrees C, and 37 degrees C but to site II only at 0 degrees C and 25 degrees C. At 37 degrees C, the temperature essential for the initiation of SV40 DNA replication in vitro, ATP is required for the interaction of T antigen and site II. ATP can be replaced efficiently by adenosine 5'-[beta,gamma-imido]triphosphate and ADP, suggesting that hydrolysis of the nucleotide is not essential for the binding of T antigen to site II. The binding to the region critical for replication can occur in the presence of a variety of nucleoside triphosphates; dATP supports binding at a concentration 1/30th that of ATP, while dGTP and rGTP were inactive at all concentrations tested.

MeSH Terms
Adenine Nucleotides/pharmacology Adenosine Triphosphate/pharmacology Animals Antigens, Polyomavirus Transforming Cell Line DNA Replication/drug effects DNA, Viral/drug effects,genetics Deoxyribonucleotides/pharmacology Genes, Viral Kinetics Simian virus 40/drug effects,genetics Structure-Activity Relationship
Chemicals
Adenine Nucleotides Antigens, Polyomavirus Transforming DNA, Viral Deoxyribonucleotides Adenosine Triphosphate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Borowiec J A
Graduate Program in Molecular Biology, Sloan-Kettering Cancer Center, New York, NY 10021.
Hurwitz J
References (31)
31 references, click to expand
  1. Variation of the average rotation angle of the DNA helix and the superhelical turns of covalently closed cyclic lambda DNA.
    J Mol Biol. 1969 Jul 14;43(1):25-39 PMID: 4897794
  2. ATP-dependent formation of a specialized nucleoprotein structure by simian virus 40 (SV40) large tumor antigen at the SV40 replication origin.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):8981-5 PMID: 2827164
  3. Protein-DNA interactions at the origin of simian virus 40 DNA replication.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:655-61 PMID: 226310
  4. Mutational analysis of the simian virus 40 replicon: pseudorevertants of mutants with a defective replication origin.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6128-31 PMID: 230499
  5. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  6. Construction and analysis of simian virus 40 origins defective in tumor antigen binding and DNA replication.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6491-5 PMID: 6256739
  7. Binding of an SV40 T antigen-related protein to the DNA of SV40 regulatory mutants.
    Nature. 1981 Feb 26;289(5800):810-3 PMID: 6258090
  8. Detection and characterization of multiple forms of simian virus 40 large T antigen.
    J Virol. 1981 Jan;37(1):92-102 PMID: 6261004
  9. Complex of simian virus 40 large tumor antigen and 48,000-dalton host tumor antigen.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):105-9 PMID: 6941238
  10. Binding of the recA protein of Escherichia coli to single- and double-stranded DNA.
    J Biol Chem. 1981 Aug 25;256(16):8835-44 PMID: 7021553
  11. T antigen binding and the control of SV40 gene expression.
    Cell. 1981 Oct;26(1 Pt 1):1-2 PMID: 6276006
  12. Territorial limits and functional anatomy of the simian virus 40 replication origin.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):381-5 PMID: 6281769
  13. DNA binding properties of simian virus 40 temperature-sensitive A proteins.
    J Virol. 1982 Nov;44(2):458-66 PMID: 6292510
  14. Topography of simian virus 40 A protein-DNA complexes: arrangement of pentanucleotide interaction sites at the origin of replication.
    J Virol. 1983 Apr;46(1):143-50 PMID: 6298451
  15. Topography of simian virus 40 A protein-DNA complexes: arrangement of protein bound to the origin of replication.
    J Virol. 1983 Apr;46(1):151-61 PMID: 6298452
  16. The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.
    Cell. 1983 Nov;35(1):79-87 PMID: 6313230
  17. The nucleotide binding site detected by affinity labeling in the large T proteins of polyoma and SV40 viruses is distinct from their ATPase catalytic site.
    J Biol Chem. 1984 Dec 25;259(24):15196-203 PMID: 6096361
  18. Monomers through trimers of large tumor antigen bind in region I and monomers through tetramers bind in region II of simian virus 40 origin of replication DNA as stable structures in solution.
    Proc Natl Acad Sci U S A. 1985 Jun;82(11):3626-30 PMID: 2987937
  19. In vitro replication of duplex circular DNA containing the simian virus 40 DNA origin site.
    Proc Natl Acad Sci U S A. 1985 Sep;82(17):5710-4 PMID: 2994044
  20. T antigen and template requirements for SV40 DNA replication in vitro.
    EMBO J. 1985 Nov;4(11):2933-9 PMID: 2998767
  21. Functional interactions of the simian virus 40 core origin of replication with flanking regulatory sequences.
    J Virol. 1986 Jan;57(1):138-44 PMID: 3001340
  22. Allosteric control of simian virus 40 T-antigen binding to viral origin DNA.
    J Virol. 1986 Jun;58(3):765-72 PMID: 3009885
  23. Simian virus 40 DNA replication in vitro: study of events preceding elongation of chains.
    Proc Natl Acad Sci U S A. 1986 Jul;83(13):4612-6 PMID: 2425351
  24. DNA helicase activity of SV40 large tumor antigen.
    EMBO J. 1986 Aug;5(8):1939-44 PMID: 3019672
  25. Functional organization of the simian virus 40 origin of DNA replication.
    Mol Cell Biol. 1986 Apr;6(4):1117-28 PMID: 3023870
  26. Simian virus 40 (SV40) DNA replication: SV40 large T antigen unwinds DNA containing the SV40 origin of replication.
    Proc Natl Acad Sci U S A. 1987 Jan;84(1):16-20 PMID: 3025851
  27. Replication of simian virus 40 origin-containing DNA in vitro with purified proteins.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1834-8 PMID: 3031654
  28. The T-antigen-binding domain of the simian virus 40 core origin of replication.
    J Virol. 1987 Jul;61(7):2143-9 PMID: 3035215
  29. Initiation of simian virus 40 DNA replication in vitro: large-tumor-antigen- and origin-dependent unwinding of the template.
    Proc Natl Acad Sci U S A. 1987 Jun;84(11):3643-7 PMID: 3035543
  30. Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8267-71 PMID: 2825183
  31. The binding site on SV40 DNA for a T antigen-related protein.
    Cell. 1978 Jan;13(1):165-79 PMID: 202398
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.1.64
Published
1988-01-00
Pages
64-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279482
Subset
IM
Grants
PHS HHS · 5R01-6M34559 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.1.64 PMC Full Text View journal details
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